Nitroblue tetrazolium chloride

Manufactured by HiMedia

Sourced in India

Nitroblue tetrazolium chloride is a chemical compound used as a laboratory reagent. It is a dark blue, crystalline powder that is soluble in water. The primary function of nitroblue tetrazolium chloride is to act as an electron acceptor in biochemical assays, particularly in the detection and quantification of enzyme activities.

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5 protocols using nitroblue tetrazolium chloride

Biochemical Characterization of Honey and Ghee

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Honey was purchased from Patanjali. Ghee was purchased from Anik. SGOT, SGPT, ALP, urea, Creatinine, BUN, glucose estimation kits were purchased from Accurex Biomedical PVT LTD., Biosar, Thane. Lipase substrate, 4-nitrophenyl butyrate, DPP-4 substrate gly-pro-p-nitroanilide, and ABTS⁺ tablet were purchased from Sigma-Aldrich. GLP-1 and GIP EIA kit were purchased from Sigma-Aldrich. Nitroblue tetrazolium chloride, metaphosphoric acid (HPO3)n, Methionine, Riboflavin, Bradford reagent, thiobarbituric acid, Bromocresol green (BCG) reagent, Trichloro-acetic acid were purchased from Hi-media Ltd, Calcutta, Hemocord-D, a hemoglobin reagent was purchased from Coral Clinical System, Goa.

Aditi P., Srivastava S., Pandey H, & Tripathi Y.B. (2020). Toxicity profile of honey and ghee, when taken together in equal ratio. Toxicology Reports, 7, 624-636.

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Oxidative Stress Measurement Protocol

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Ferric chloride, hydrogen peroxide, phosphate buffer saline (PBS), bicarbonate buffer, thiobarbituric acid (TBA), and trichloroacetic acid (TCA) were obtained from sigma chemical company. Nitroblue tetrazolium chloride (Hi Media), Dialysis membrane (Hi Media), BCA estimation Kit (G Biosciences), and 96 well plates (Nunc) were obtained. The other reagents used were tri-sodium citrate buffer, 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride and 9,10-phenanthrenequinone, Tween-20, anti-rabbit IgG, dialysis tubing, and p-nitrophenyl phosphate. All other chemicals used in this study were of analytical grade available.

Alouffi S., Faisal M., Alatar A.A, & Ahmad S. (2018). Oxidative Modification of LDL by Various Physicochemical Techniques: Its Probable Role in Diabetes Coupled with CVDs. BioMed Research International, 2018, 7390612.

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Comprehensive Biochemical Protocol Compendium

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DMBA (7, 12- dimethylbenz(α)anthracene), Indole 3 carbinol (I3C), 5-5′dithiobisnitrobenzoic acid (DTNB) sodium dithionate and malondialdehyde were obtained from Sigma-aldrich, Banglore, India. Xylenol orange, ammonium ferrous sulfate, cyclohexane, 2,6-dichlorophenol-indophenol (DCPIP), reduced nicotinamide adenine dinucleotide (NADH), reduced nicotinamide adenine dinucleotide phosphate (NADPH), bovine serum albumin (BSA), potassium ferricyanide, 1-chloro-2,4-dinitrobenzene (CDNB), glycylglycine, reduced glutathione (GSH), oxidized glutathione (GSSG), ascorbic acid, guiacol, triton X, nitroblue tetrazolium chloride (NBT), hydroxylamine hydrochloride, pyruvate were obtained from Himedia Laboratories, Mumbai, India. All other reagents used in the present study were of analytical grade.

Kumar R., Kaur R., Singh A.P, & Arora S. (2014). Diminution of Hepatic Response to 7, 12-dimethylbenz(α)anthracene by Ethyl Acetate Fraction of Acacia catechu Willd. through Modulation of Xenobiotic and Anti-Oxidative Enzymes in Rats. PLoS ONE, 9(2), e90083.

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Adipogenic Differentiation Assay Protocol

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Syringic acid (SA, Sigma), Dulbecco's Modified Eagle's Medium (DMEM, HiMedia), phenol red-free Dulbecco's modified eagle's medium (HiMedia), insulin (HiMedia), dexamethasone (DEX, HiMedia), 0.25% trypsin-EDTA (HiMedia), Dulbecco's Phosphate Buffered Saline (DPBS, HiMedia), 3-isobutyl-1-methylxanthine (IBMX, Sigma), dimethyl sulphoxide (DMSO), free glycerol reagent (Sigma), foetal bovine serum (FBS, Gibco®), 100X antibiotic-antimycotic (Gibco®), thiazolyl blue tetrazolium bromide (MTT, HiMedia), β-nicotinamide adenine dinucleotide (reduced) disodium salt (β-NADH, SRL Pvt. Ltd), Oil-Red-O (ORO, HiMedia), nitroblue tetrazolium chloride (NBT, HiMedia), fatty acid-free bovine serum albumin (BSA, HiMedia) . All reagents and chemicals used were of tissue culture grade.

John C.M, & Arockiasamy S. (2020). Syringic acid (4-hydroxy-3,5-dimethoxybenzoic acid) inhibits adipogenesis and promotes lipolysis in 3T3-L1 adipocytes. Natural product research, 34(23).

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Antioxidant Enzyme Activity Assay

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Dichloromethane, ethyl acetate, hexane, sodium bicinchoninate, 7, 12-dimethylbenz(α)anthracene, 5-5-dithiobisnitrobenzoic acid (DTNB), sodium dithionate, L-γ-glutamyl-4-nitroanilide, H₂O₂, and malondialdehyde (MDA) were purchased from Sigma-aldrich, Bangalore, India. Xylenol orange, ammonium ferrous sulfate, cyclohexane, 2, 6-dichlorophenol-indophenol (DCPIP), reduced nicotinamide adenine dinucleotide (NADH), reduced nicotinamide adenine dinucleotide phosphate (NADPH), bovine serum albumin (BSA), potassium ferricyanide, 1-chloro-2,4-dinitrobenzene (CDNB), glycylglycine, reduced glutathione (GSH), oxidized glutathione (GSSG), ascorbic acid, guaiacol, Triton-X, nitroblue tetrazolium chloride (NBT), hydro-xylamine hydrochloride, and pyruvate were purchased from Himedia Laboratories, Mumbai, India. DMBA (7, 12- dimethylbenz(α)anthracene) was obtained from Sigma-aldrich, Bangalore, India. Autopack kit for analyzing bilirubin was purchased from Beacon Diagnostics, Gujarat, India, while alkaline phosphatase, serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase autopack kits were purchased from Delta Labs, Sindhudurg, India. Rest of the chemicals used in the present study were of analytical grade.

Arora R., Bhushan S., Kumar R., Mannan R., Kaur P., Singh A.P., Singh B., Vig A.P., Sharma D, & Arora S. (2014). Hepatic Dysfunction Induced by 7, 12-Dimethylbenz(α)anthracene and Its Obviation with Erucin Using Enzymatic and Histological Changes as Indicators. PLoS ONE, 9(11), e112614.

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