Resveratrol (RSV), LPS, compound C (CC), and Ficoll-Paque were purchased from Sigma-Aldrich (St. Louis, MO, USA). Recombinant human IL-4, recombinant human macrophage-colony-stimulating factor (M-CSF), and interferon (INF) γ were from Pepro Tech (Rocky Hill, NJ, USA). Sirtinol was obtained from Calbiochem (La Jolla, CA, USA). Antibodies specific for AMPKα, phosphor-AMPKα, phosphor-NF-κB p65 (Lys310), acetyl-NF-κB p65 (Lys310), acetyl-CoA carboxylase (ACC), and phosphor-ACC were from Cell Signaling (Danvers, MA, USA). Antibodies specific for SIRT1, NF-κB p65, ariginase-1, and Histone H1 were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Antibodies specific for TNFα and IL-1β were from Abcam (Cambridge, UK). Antibody specific for TRANCE/TNFSF11 was from R&D Systems (Minneapolis, MN, USA).
Phosphor acc
Manufactured by Cell Signaling Technology
Sourced in United States
Phosphor-ACC is a laboratory product from Cell Signaling Technology. It is designed to detect and quantify the phosphorylation of acetyl-CoA carboxylase (ACC) protein. The core function of Phosphor-ACC is to provide a tool for researchers to analyze the activation state of this important metabolic enzyme.
Automatically generated - may contain errors
Lab products found in correlation
Metformin, by Merck Group (2 mentions)
Bca protein assay, by Thermo Fisher Scientific (2 mentions)
Recombinant human macrophage colony stimulating factor m csf, by Thermo Fisher Scientific (1 mentions)
Ficoll paque, by Merck Group (1 mentions)
Recombinant human il 4, by Thermo Fisher Scientific (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Rabbit anti mouse igg, by Merck Group (1 mentions)
Phosphor ampk, by Cell Signaling Technology (1 mentions)
Phospho creb, by Abcam (1 mentions)
Hrp conjugated secondary ab, by Merck Group (1 mentions)
Phospho mtor, by Cell Signaling Technology (1 mentions)
β actin, by Abcam (1 mentions)
Aspirin, by Merck Group (1 mentions)
Phosphor mtor, by Cell Signaling Technology (1 mentions)
Interleukin 6 il 6, by Merck Group (1 mentions)
Cox 2, by Cell Signaling Technology (1 mentions)
Phosphor 4e bp1, by Cell Signaling Technology (1 mentions)
Phosphor ampkα, by Cell Signaling Technology (1 mentions)
Phosphor stat3, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
5 protocols using phosphor acc
1
Resveratrol Modulates Macrophage Activation
2
Oleanolic Acid Modulates Glucose Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oleanolic acid (structure shown in Figure 1 ) was obtained from the Center for Drug Discovery at China Pharmaceutical University. Metformin (purity > 98%) was obtained from Sigma (St. Louis, MO, USA). The mouse insulin enzyme immunoassay ELISA kit was purchased from Invitrogen. Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) kits were purchased from Whitman Biotech (Nanjing, Ltd., China). Antibody (Ab) for G-6-Pase was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). TORC2 was from Millipore (Billerica, MA, USA), and phospho-CREB and β-actin were from Abcam (Cambridge, MA, USA). The Abs against protein kinase B (PKB/Akt), phospho-Ser473-Akt (pAkt), PI3K, phospho-PI3K, AMPK, phosphor-AMPK, ACC, phosphor-ACC, PEPCK1, mTOR, and phospho-mTOR were purchased from Cell Signaling Technology (Beverly, MA, USA). The HRP-conjugated secondary Abs, affinity-purified mouse anti-rabbit IgG, and rabbit anti-mouse IgG were purchased from Sigma (St. Louis, MO, USA).
3
Immunoblotting for Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against phosphor-AMPKα, AMPKα, phosphor-ACC, ACC, phosphor-mTOR, mTOR, phosphor-p70S6K, p70S6K, phosphor-4E-BP1, 4E-BP1, phorphor-NFκB p65, NFκB p65, IκBα, COX2, phosphor-STAT3, STAT3, Mcl-1, Bcl-xL, Bim, Bak, Bax, Bid, Puma and Bad were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against Bcl-2 and Protein A/G Agarose beads were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Metformin and aspirin as well as interleukin-6 (IL-6) were purchased from Sigma-Aldrich (St Louis, MO, USA). AZD-8055 was purchase from Invitrogen (Carlsbad, CA, USA).
4
Adipogenesis Inhibition Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Penicillin-streptomycin (cat. LS 202-02), Trypsin-EDTA solution (cat. LS 015-08), and Dulbecco’s modified eagle medium (DMEM) (cat. LM 001-05) were purchased from WelGENE (Seoul, Republic of Korea). YM976 (cat. Y4877), Compound C (cat. 171260), Oil Red O (cat. O0625), dexamethasone (cat. D4902), dimethyl sulfoxide (DMSO, cat. D8418), IBMX (cat. I5879), 10% formalin (cat. HT501128), and insulin (cat. I0516) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against phospho-AMPK (cat. 2535), AMPKα (cat. 2532), C/EBPα (cat. 2295), FASN (cat. 3189), FABP4 (cat. 2120), phospho-CREB (cat. 9198), CREB (cat. 9197), phosphor-ACC (cat. 3661), and ACC (cat. 3662) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against PPARγ (cat. sc-7273) and AMPKα siRNA (cat. sc-29674) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Fetal bovine serum (FBS, cat. 12483020), bovine serum (cat. 16170078), and ECL Western Blotting Substrate (cat. 32106) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Protease inhibitor cocktail (cat. 11697498001) was purchased from Roche Diagnostics (Indianapolis, IN, USA).
5
Western Blot Analysis of Metabolic Proteins
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!