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Lineal accelerator

Manufactured by Siemens
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The Siemens lineal accelerator is a medical device designed for the delivery of high-energy radiation therapy. It generates and directs a beam of ionizing radiation, typically X-rays or electrons, towards a specific target area in the patient's body. The device is used in the treatment of various types of cancer and other medical conditions.

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Lab products found in correlation

Pi apoptosis detection kit 1, by BD (2 mentions) Facscalibur flow cytometer, by BD (2 mentions)

2 protocols using lineal accelerator

1

Radioresistant Cell Apoptosis Analysis

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B16-F1 and Cloudman cells were γ-irradiated (70 Gy, Siemens lineal accelerator, Instituto Alexander Fleming, Buenos Aires) and stored in liquid nitrogen. Cells were thawed and cultured using the corresponding culture media for each cell line. Non-adherent and adherent cells were collected. Apoptosis and necrosis were determined with FITC-Annexin V (AnV) – propidium iodide (PI) Apoptosis Detection Kit I (BD Biosciences) following the manufacturer’s recommendations, measured by flow cytometry (FACSCalibur Flow Cytometer, Becton Dickinson) and analyzed using FlowJo 7.6 software.
Keon S.M., Bentivegna S., Levy E.M., Marks M.S., Mantegazza A.R., Wainstok R, & Mordoh J. (2019). Syngeneic B16-F1 cells are more efficient than allogeneic Cloudman cells as antigen source in DC-based vaccination in the B16-F1 murine melanoma model. Vaccine, 37(35), 4947-4955.
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2

Radioresistant Cell Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
B16-F1 and Cloudman cells were γ-irradiated (70 Gy, Siemens lineal accelerator, Instituto Alexander Fleming, Buenos Aires) and stored in liquid nitrogen. Cells were thawed and cultured using the corresponding culture media for each cell line. Non-adherent and adherent cells were collected. Apoptosis and necrosis were determined with FITC-Annexin V (AnV) – propidium iodide (PI) Apoptosis Detection Kit I (BD Biosciences) following the manufacturer’s recommendations, measured by flow cytometry (FACSCalibur Flow Cytometer, Becton Dickinson) and analyzed using FlowJo 7.6 software.
Keon S.M., Bentivegna S., Levy E.M., Marks M.S., Mantegazza A.R., Wainstok R, & Mordoh J. (2019). Syngeneic B16-F1 cells are more efficient than allogeneic Cloudman cells as antigen source in DC-based vaccination in the B16-F1 murine melanoma model. Vaccine, 37(35), 4947-4955.
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