Lemongrass essential oil (Cymbopogon Schoenanthus Oil, reagent grade, 100%) was purchased from Spectrum Chemical MFG Corp (New Brunswick, NJ, USA). Tween 80 (P1754) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). Aspergillus flavus Link 200026 NRRL 3357 strains purchased from ATCC (Manassas, VA, USA) were used. All other chemicals and supplies were purchased from VWR (Radnor, PA, USA) or Fisher Scientific (Pittsburgh, PA, USA). Corn stover-derived TEMPO-CNF was prepared according to our previous publication [23 (link)]. Specifically, corn stover was ground to a size below 1/8 inch, followed by washing to remove impurities. Then, corn stover was bleached to remove lignin, followed by alkaline treatment to remove hemicellulose. Lastly, corn stover went through TEMPO oxidation and sonication to achieve TEMPO-CNF. Corn stover-derived TEMPO-CNF had a length of 353 nm, width of 4 nm, surface charge of 1.48 ± 0.09 mmol/g COO− and zeta potential of −65 ± 3 mV [23 (link)]. The as-prepared TEMPO-CNF gel had a solid content of ~3.8 wt% [23 (link)].
Tween 80 p1754
Manufactured by Merck Group
Sourced in United States
Tween 80 (P1754) is a non-ionic surfactant used in various laboratory applications. It is a polyoxyethylene sorbitan monooleate compound that can be utilized as an emulsifier, dispersing agent, and wetting agent in diverse experimental setups.
Automatically generated - may contain errors
Lab products found in correlation
Aspergillus flavus link 200026 nrrl 3357 strains, by American Type Culture Collection (2 mentions)
Carvacrol, by Merck Group (1 mentions)
Anti rabbit hrp secondary, by Cell Signaling Technology (1 mentions)
Gapdh, by Abcam (1 mentions)
Midazolam hydrochloride, by Akorn (1 mentions)
Zetasizer nano zs instrument, by Malvern Panalytical (1 mentions)
Jem 2100, by JEOL (1 mentions)
Sodium chloride (nacl), by Avantor (1 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (1 mentions)
7 protocols using tween 80 p1754
1
Corn Stover-Derived TEMPO-CNF Preparation
2
Corn Stover-Derived TEMPO-CNF Preparation
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Lemongrass essential oil (Cymbopogon Schoenanthus Oil, reagent grade, 100%) was purchased from Spectrum Chemical MFG Corp (New Brunswick, NJ, USA). Tween 80 (P1754) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). Aspergillus flavus Link 200026 NRRL 3357 strains purchased from ATCC (Manassas, VA, USA) were used. All other chemicals and supplies were purchased from VWR (Radnor, PA, USA) or Fisher Scientific (Pittsburgh, PA, USA). Corn stover-derived TEMPO-CNF was prepared according to our previous publication [23 (link)]. Specifically, corn stover was ground to a size below 1/8 inch, followed by washing to remove impurities. Then, corn stover was bleached to remove lignin, followed by alkaline treatment to remove hemicellulose. Lastly, corn stover went through TEMPO oxidation and sonication to achieve TEMPO-CNF. Corn stover-derived TEMPO-CNF had a length of 353 nm, width of 4 nm, surface charge of 1.48 ± 0.09 mmol/g COO− and zeta potential of −65 ± 3 mV [23 (link)]. The as-prepared TEMPO-CNF gel had a solid content of ~3.8 wt% [23 (link)].
3
Oleic Acid-Based Nanoemulsion Formulation
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Oleic acid pharma grade (142659.1611) with purity 88% is purchased from Panreac. Synthetic non-ionic surfactant Tween 80 ® (P1754) and potassium hydroxide pellets 85% A.C.S Reagent is purchased from Sigma Aldrich. Carvacrol (Merck W224502) with purity >98% food grade is used. Medium chain triglycerides (MCT) oil (Miglyol 812) is purchased from Acofarma. The manufacturer reported a composition of 58.1% of caprylic acid (C8:0) and 41.9% of capric acid (C10:0). Water is deionized and further purified by mili-Q filtration.
4
Intranasal Administration of GL-II-73 in Rats
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The proprietary compound GL-II-73 was supplied by the Centre for Addiction and Mental Health, Campbell Family Mental Health Research Institute (Toronto, ON, Canada). GL-II-73 or vehicle (85% H20, 14% propylene glycol, 1% Tween80) was administered directly into the vHipp (100 ng/uL; 0.75 µL; AP −5.3, ML ± 5.0, DV −6.0 mm from bregma) 20 minutes prior to electrophysiology or behavior. This dose and timing was selected based on previous characterization (Prevot et al., 2019 (link)) as well as our own data (Perez et al., 2022 (link)). Although systemic administration would be a more therapeutically relevant approach, we chose intra-vHipp administration because rats seem to rapidly metabolize this compound (Prevot et al., unpublished observations). Importantly, this effect appears to be exclusive to rats, because GL-II-73 can be administered systemically to mice and achieves sufficient brain concentrations to produce robust behavioral effects (Prevot et al., 2019 (link)). Chloral hydrate (C8383), propylene glycol (P4347), and Tween80 (P1754) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
5
Pharmacological Modulation of α5-GABAA Receptors
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The proprietary compound, GL-II-73, was supplied by the Centre for Addiction and Mental Health, Campbell Family Mental Health Research Institute (Toronto, ON, Canada). Pilocarpine hydrochloride (P6503), chloral hydrate (C8383), propylene glycol (P4347), DPX (06522) and Tween80 (P1754) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Midazolam hydrochloride was purchased from Akorn Pharmaceuticals (Lake Forest, IL, USA). The α5 antibody was from R&D Systems (#PPS027; Minneapolis, MN USA) and the anti-rabbit-HRP secondary was from Cell signaling (#7074; Danvers, MA, USA). All other antibodies were from Abcam (Cambridge, UK), including gephryin (ab181382) and GAPDH (#9484). Dynabeads protein G magnetic beads (10003D), ECL substrate (32106), stripping buffer (21059), and coverslips (174942) were from ThermoFisher (Waltham, MA, USA). Gels (4569035) and membranes (1704158) were from Bio-Rad. All other chemicals or reagents were of either analytical or laboratory grade and purchased from various suppliers.
6
Synthesis of Soybean Stover-Derived CNC
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Lemongrass essential oil (Cymbopogon Schoenanthus Oil, reagent grade, 100%) was obtained from Spectrum Chemical MFG Corp (New Brunswick, NJ, USA). Tween 80 (P1754) was obtained from Sigma-Aldrich, Inc. (St. Louis, MO, USA). All other chemicals were acquired from VWR (Radnor, PA, USA) or Fisher Scientific (Pittsburgh, PA, USA). Aspergillus flavus Link 200026 NRRL 3357 strain was acquired from ATCC (Manassas, VA, USA). Soybean stover-derived CNC was made following our previously published method [20 (link)]. Firstly, soybean stover was collected from the farm, ground to below 1/8 of an inch, then washed for the removal of impurities. Following this, soybean stover was treated with an alkaline solution to mainly remove hemicellulose. After alkaline treatment, samples underwent a bleaching process to mainly eliminate lignin. Lastly, sulfuric acid hydrolysis and sonication were used on soybean stover to obtain soybean CNC. Soybean stover-derived CNC had a length of 117 ± 40 nm and width of 7.3 ± 2.0 nm as characterized by transmission electron microscopes (JEM-2100, JEOL Ltd., Akishima, Tokyo, Japan), with a mean particle size of 197 ± 6 nm, a polydispersity index of 0.65, and a zeta potential of −50 ± 1 mV as characterized by a Zetasizer Nano ZS instrument (Malvern Instruments Ltd., Worcestershire, UK).
7
Soybean Stover Valorization Protocol
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Soybean stover was obtained from Iowa State University Research Farms (Boone, IA, USA) and reduced to a 1/8″ size using a knife mill. Lemongrass essential oil (Cymbopogon schoenanthus Oil, 100%, reagent grade) was obtained from Spectrum Chemical MFG Corp (New Brunswick, NJ). Tween 80 (P1754) (Sigma-Aldrich, Inc. (St. Louis, MO, USA), sulfuric acids (95–98%) (chemPUR, Karlsruhe, Germany), glacial acetic acids (Fisher scientific, Hampton, NH, USA), sodium hydroxide (Macron Fine Chemicals, Center Valley, PA, USA), sodium chlorite (Beantown chemical, Hudson, NH, USA), sodium chloride (VWR International, Radnor, PA, USA) and dialysis tubing (Cellulose, 12–14 kDa MWCO) (Chemical MFG Corp, New Brunswick, NJ, USA) were used in this study.
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