Vglut3 ires cre

Manufactured by Jackson ImmunoResearch

VGLUT3::IRES-Cre is a genetic targeting tool that allows for the specific labeling and manipulation of neurons expressing the vesicular glutamate transporter 3 (VGLUT3) protein. It consists of an internal ribosome entry site (IRES) that allows for the co-expression of both VGLUT3 and the Cre recombinase enzyme from a single mRNA transcript.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using vglut3 ires cre

Genetic Mouse Lines for Neuroscience Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following mouse lines (25-30 g, 8-12 weeks old, male) were used for the experiments: C57Bl6 mice (Jackson Laboratory), DAT::IRES-Cre (Jackson Laboratory, stock number: 006660, strain code: B6.SJL-Slc6a3tm1.1(cre)Bkmn/J), VGLUT2::IRES-Cre (Jackson Laboratory, stock number: 016963, strain code: Slc17a6tm2(cre)Lowl/J), VGLUT3::IRES-Cre (Jackson Laboratory, stock number: 018147, strain code: Tg(Slc17a8-icre)1Edw/SealJ), GAD2::IRES-Cre (Jackson Laboratory, stock number: 010802, strain code: Gad2^tm2(cre)Zjh/J), Ai14 Cre reporter mice (Jackson Laboratory, stock number: 007908, strain code: B6;129S6 Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J). Ai14 Cre reporter mice were crossed to GAD2::IRES-Cre and VGLUT2::IRES-Cre mice. Mice were maintained on a 12:12 light cycle (lights on at 07:00). All procedures complied with the animal care standards set forth by the National Institutes of Health and were approved by University of California Berkeley’s Administrative Panel on Laboratory Animal Care.

de Jong J.W., Afjei S.A., Dorocic I.P., Peck J.R., Liu C., Kim C.K., Tian L., Deisseroth K, & Lammel S. (2018). A neural circuit mechanism for encoding aversive stimuli in the mesolimbic dopamine system. Neuron, 101(1), 133-151.e7.

+ Open protocol

+ Expand

Comprehensive Murine Metabolic Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental approaches were approved by The Rockefeller University Institutional Animal Care and Use Committee protocol number 18066-H and were in accordance with the National Institutes of Health guidelines. Adult mice (>8 weeks old) were used for all studies. Mice were housed in a 12-hour light–dark cycle (7:00–19:00) with ad libitum access to food and water unless otherwise indicated (fasting studies and HFD studies). All lines are in a wild-type (C57BL/6J) background. Genotypes and sources for mice used in the above studies are: C57Bl6J (Jackson Laboratory 000664), Ob/ob (Jackson Laboratory 000632), Vglut3-IRES-Cre (gift from B. Lowell), Vglut2-IRES-Cre (Jackson Laboratory 016963). Male mice were used for molecular studies. Male and female mice were used for physiology studies.

Schneeberger M., Brice N.L., Pellegrino K., Parolari L., Shaked J.T., Page K.J., Marchildon F., Barrows D.W., Carroll T.S., Topilko T., Mulligan V.M., Newman R., Doyle K., Bürli R., Barker D.F., Glen A., Ortuño M.J., Nectow A.R., Renier N., Cohen P., Carlton M., Heintz N, & Friedman J.M. (2022). Pharmacological targeting of glutamatergic neurons within the brainstem for weight reduction. Nature Metabolism, 4(11), 1495-1513.

+ Open protocol

+ Expand

Mouse Cre Line Characterization

Cited 5 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phox2b-Cre (Scott et al., 2011 (link)) (JAX# 016223), Wnt1-Cre (Chai et al., 2000 (link)) (JAX# 009107), Trpv1-ires-Cre (Cavanaugh et al., 2011 (link)) (JAX# 017769), Tac1-ires-Cre (Harris et al., 2014 (link)) (JAX# 021877), Sst-ires-Cre (Taniguchi et al., 2011 (link)) (JAX# 013044), Vglut3-ires-Cre (Tasic et al., 2018 (link)) (JAX# 028534), Glp1r-ires-Cre (Williams et al., 2016 (link)) (JAX# 029283), Gpr65-ires-Cre (Williams et al., 2016 (link)) (JAX# 029282) were previously described and purchased from Jackson Laboratories.

Borgmann D., Ciglieri E., Biglari N., Brandt C., Cremer A.L., Backes H., Tittgemeyer M., Wunderlich F.T., Brüning J.C, & Fenselau H. (2021). Gut-brain communication by distinct sensory neurons differently controls feeding and glucose metabolism. Cell Metabolism, 33(7), 1466-1482.e7.

+ Open protocol

+ Expand

Cre-Expressing Mouse Models for Neuroscience

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male 8–12-week old, vGAT-iRES-Cre (Jackson Laboratory, JAX:016962) and vGlut3-iRES-Cre (Jackson Laboratory, JAX:018147) mice were used in the present study. Animals, two to three mice per cage, were kept in temperature- and humidity-controlled housing facilities (22 °C and 50 ± 5%) with a standard 12-h light–dark cycle, with food and water available ad libitum. All experiments were approved by the Animal Care and Use Committee of the Institute of Experimental Medicine and the Committee for Scientific Ethics of Animal Research of the National Food Chain Safety Office under the project number PE/EA/200-2/2020 and were performed according to the 2010/63/EU Directive of the EC Council. All efforts were made to minimize pain and suffering and to reduce the number of animals used.

Barth A.M., Jelitai M., Vasarhelyi-Nagy M.F, & Varga V. (2023). Aversive stimulus-tuned responses in the CA1 of the dorsal hippocampus. Nature Communications, 14, 6841.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!