Rabbit anti mouse igg conjugated with horseradish peroxidase

Manufactured by Thermo Fisher Scientific

Rabbit anti-mouse IgG-conjugated with horseradish peroxidase is a secondary antibody used in immunoassays and immunochemical techniques. It is designed to bind to mouse primary antibodies, with the horseradish peroxidase enzyme conjugated to facilitate signal detection.

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Lab products found in correlation

Gapdh, by Santa Cruz Biotechnology (2 mentions) Kcnq1, by Santa Cruz Biotechnology (1 mentions) Tgfβ2, by Cell Signaling Technology (1 mentions) Serca2a, by Santa Cruz Biotechnology (1 mentions) Cl xposure film, by Thermo Fisher Scientific (1 mentions) Ecl western blotting substrate, by Bio-Rad (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Anti histone 3, by Cell Signaling Technology (1 mentions) P70s6k1, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Horseradish peroxidase (215 citations) IgG Rabbit (4 citations) Mouse anti-IgG (2 citations)

2 protocols using rabbit anti mouse igg conjugated with horseradish peroxidase

Protein Expression Analysis by Western Blotting

Cited 1 time

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Total protein was separated by SDS-PAGE gel, and transferred onto a PVDF membrane. The membrane was probed with antibodies against FHL (Santa Cruz), CRP2 (Santa Cruz), IGFBP8/CTGF (Abcam), KCNQ1 (Santa Cruz), TGF β2 (Cell Signaling), RyR2 (Abcam), SERCA2A (Santa Cruz), TRDN (Santa Cruz), CASQ1 (Santa Cruz), PLB (Abcam), Rabbit anti-mouse IgG-conjugated with horseradish peroxidase (Fisher Scientific) was served as the secondary antibody. GAPDH (Santa Cruz) was served as the protein loading control. The detailed procedure was described previously 34 (link).

Guo W., Zhu C., Yin Z., Wang Q., Sun M., Cao H, & Greaser M.L. (2018). Splicing Factor RBM20 Regulates Transcriptional Network of Titin Associated and Calcium Handling Genes in The Heart. International Journal of Biological Sciences, 14(4), 369-380.

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Western Blot Analysis of RBM20 and Signaling

Cited 2 times

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Total protein was separated by SDS-PAGE gel, and transferred onto a PVDF membrane. The membrane was probed with antibodies against RBM20 (home-made) which was validated in our previous studies [9 , 18 (link), 27 (link), 43 (link)], Akt (Cell Signaling), phospho(p)-Akt(Ser473) (Cell Signaling), p70S6K1(Cell Signaling), phosphor(p)-p70S6K1 (Thr389) (Cell Signaling), 4E-BP1 (Cell Signaling). Rabbit anti-mouse IgG-conjugated with horseradish peroxidase (Fisher Scientific) was served as the secondary antibody. The blot was developed with ECL western blotting substrate (Bio-Rad) and exposed to CL-Xposure film (Thermo Scientific). Anti-Histone3 (Cell Signaling) and GAPDH (Santa Cruz) were served as the protein loading control.

Zhu C., Yin Z., Tan B, & Guo W. (2017). Insulin regulates titin pre-mRNA splicing through the PI3K-Akt-mTOR kinase axis in a RBM20-dependent manner. Biochimica et biophysica acta, 1863(9), 2363-2371.

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