Mouse anti human p53

Manufactured by Cell Signaling Technology

Mouse anti-human p53 is a primary antibody that recognizes the p53 protein, a key regulator of cellular processes such as cell cycle, apoptosis, and DNA repair. This antibody is suitable for use in various immunodetection techniques, including Western blotting, immunoprecipitation, and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti human 53bp1, by Cell Signaling Technology (1 mentions) Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Phalloidin, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 568 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Image pro plus, by Media Cybernetics (1 mentions) Mouse anti β actin, by Cell Signaling Technology (1 mentions) Cell culture media, by Thermo Fisher Scientific (1 mentions) Cisplatin, by Merck Group (1 mentions)

3 protocols using mouse anti human p53

Immunofluorescence Analysis of DNA Damage Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse monoclonal anti-human Phospho-histone H2AX (cat. no. 05-636; 1:1,000 dilution; Merck Millipore), rabbit anti-human histone H2AX (cat. no. P16104; 1:2,000 dilution; Ray Biotech), rabbit anti-human p21 (cat. no. 2147; 1:1,000 dilution; Cell Signaling Technology), rabbit anti-human 53BP1 (cat. no. 4937; 1:1,000 dilution; Cell Signaling Technology), mouse anti-human p53 (cat. no. 48818; 1:1,000 dilution; Cell Signaling Technology), mouse monoclonal anti-gizzard β-actin (cat. no. sc-47778; 1:5,000 dilution; Santa Cruz Biotechnology), phalloidin (cat. no. A12379; 1:1,000 dilution; Thermo Fisher Scientific Inc.), DAPI (cat. no. D1306; 1:36,000 dilution; Thermo Fisher Scientific Inc.). Secondary antibodies used are goat anti-mouse Alexa Fluor 568 (A11004; 1:1,000 dilution; Thermo Fisher Scientific, Inc.), goat anti-rabbit Alexa Fluor 488 (cat. no. A11034; 1:1,000 dilution; Thermo Fisher Scientific Inc.), goat anti-mouse Alexa Fluor 488 (cat. no. A11001; 1:1,000 dilution; Thermo Fisher Scientific Inc.), goat anti-rabbit Alexa Fluor 568 (cat. no. A11011; 1:1.000 dilution; Thermo Fisher Scientific Inc.).

Zaarour R.F., Prasad P., Venkatesh G.H., Khouzam R.A., Amirtharaj F., Zeinelabdin N., Rifath A., Terry S., Nawafleh H., El Sayed Y, & Chouaib S. (2021). Waterpipe smoke condensate influences epithelial to mesenchymal transition and interferes with the cytotoxic immune response in non-small cell lung cancer cell lines. Oncology Reports, 45(3), 879-890.

+ Open protocol

+ Expand

Immunohistochemical Detection of LIN28B and p53

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed using the VECTASTAIN ABC Kit (Vector). The following primary antibodies were used in this study: rabbit anti-human LIN28B (1:400) and mouse anti-human p53 (1:1800) (both from Cell Signaling Technology). Antibodies were incubated overnight at 4 °C and the immunoreaction was visualized using 3,3′-diaminobenzidine. Images were collected and analyzed using Image-Pro Plus software (Media Cybernetics).

Shi J., Jin X., Wang Y., Zhu T., Zhang D., Li Q., Zhong X., Deng Y., Shen J, & Fan X. (2022). LIN28B inhibition sensitizes cells to p53-restoring PPI therapy through unleashed translational suppression. Oncogenesis, 11(1), 37.

+ Open protocol

+ Expand

Radiolabeling and Characterization of p53

Check if the same lab product or an alternative is used in the 5 most similar protocols

⁶⁴Cu (t_1/2 = 12.7 hours, β⁺; 17.8%, E_{β + max} = 656 KeV, β^-, 38.4%, E_{β- max} = 573 KeV) was obtained from Washington University (St. Louis, MO) and University of Wisconsin (Madison, WI). All chemicals and solvents were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO), unless otherwise specified. Cell culture media were purchased form Invitrogen (Grand Island, NY). Cisplatin was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO). Aqueous solutions were prepared using ultrapure water (resistivity, 18 M). Mouse Anti-Human p53 and mouse anti-β-Actin were purchased from cell signaling (Danvers, MA). Mouse Anti-Human p53 (PAb240) and mouse Anti-Human Atox1 were purchased from Abcam (Cambridge, MA), and mouse Anti-Human TBP was purchased from Pierce (Rockford, IL).

Beaino W., Guo Y., Chang A.J, & Anderson C.J. (2014). Roles of Atox1 and p53 in the trafficking of copper-64 to tumor cell nuclei: implications for cancer therapy. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 19(3), 427-438.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!