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5 protocols using r pulegone

1

Quantifying Antioxidant Potential of Terpenes

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The terpenes (−)-isopulegol (≥99%), menthol (≥99%), p-cymene (≥99%), eucalyptol (≥99%), (R)-(+)-pulegone (97%), γ-terpinene (97%), α-terpinene (≥95%), linalool (≥97%), (S)-(+)-carvone (≥96%), citronellal (≥95%), (−)-terpinene-4-ol (≥95%), citral (≥95%), menthone (≥90%), farnesene (mixtures of isomers, ≥90%), α-phellandrene (≥90%), β-myrcene (≥90%), and TPTZ (2,4,6-Tri(2-pyridyl)-s-triazine)) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid (ACS), FeCl3 × 6H2O, FeSO4 × 7H2O, phosphoric acid (ACS), hydrochloric acid (ACS), chloroform (ACS), ethyl acetate (ACS), 2-butanone (ACS), and methanol (analytic purity grade) were obtained from Polish Reagents (Gliwice, Poland). The standards trolox ((±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, 97%) and gallic acid (>98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Essential Oils and Chromatographic Standards

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1,8-Cineole, citronellal, citronellic acid, citronellol, linalool, (R)-limonene, (R)-α-pinene, (S)-β-pinene, (R)-pulegone, α-terpinene, γ-terpinene, thymol and PBO used in the bioassays or as chromatographic standards were purchased from Sigma-Aldrich (St. Louis, MO, United States). HPLC grade acetone was purchased from Merck (Darmstadt, Germany).
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3

Insecticidal Efficacy of Medicinal Plants

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To evaluate the insecticidal effect, Mentha pulegium and Rosmarinus officinalis were collected during their flowering period (between July and September for M. pulegium and between January and May for R. officinalis) from a mountainous zone in Taounate town (northeastern Morocco). The samples were identified at the National Agency for Medicinal and Aromatic Plants in Taounate, Morocco. The pure compounds; 1,8-cineole (99%), camphor (98%), α-pinene (98%), carvone (98%) and R(+)-pulegone (99%) were purchased from Sigma-Aldrich (Steinheim, Germany). Deltamethrin (0.05%) was used as a positive control at the diagnostic dose suggested by the World Health Organization for mosquito adults.
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4

Mosquito Adulticide Evaluation Protocol

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The pure compounds 1,8-cineole (99%), α-pinene (98%), Carvone (98%), and R (+)-pulegone (99%) (Figure 1) were purchased from Sigma-Aldrich (Darmstadt, Germany). Deltamethrin, obtained from the Scientific Fertilizer Co. (P) Ltd. (Coimbatore, India), was used as a positive control at the diagnostic dose (0.05%) suggested by the World Health Organization (Geneva, Switzerland) for adult mosquitoes.
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5

Synthesis and Characterization of Polymer Electrolytes

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Vinylimidazole (99%), bromoacetonitrile (97%), poly(acrylic acid) (PAA) (Mw—2000 Da), and poly(N,N-dimethyldiallylammonium chloride) (Mw—400,000–500,000 Da) were purchased from Sigma Aldrich (Gillingham, United Kingdom). Lithium bis(trifluromethane sulfonyl)imide (LiTFSI, 99.95%) was purchased from Io-li-tec (Heilbronn, Germany). All chemicals were used without any further purification. Solvents were of analytical grade. Pentanoic acid (>99%), hexanoic acid (>99%), 3-pentanone (>99%), 2-hexanone (>98%), 2-heptanone (>98%), 2-methylbutanal (98%), 1-heptanal (95%), 1-hexanol (99.9%), 2–heptanol (98%), 1–octanol (99.7%), benzyl alcohol (99.8%), 1-ethylnaphtalene (>97%), carvacrol (>98%), (+)-α-pinene (>99%), α-terpineol (>99%), eucalyptol (99%), (R)-(+)-pulegone (97%), and β-citronellol (95%) were purchased from Sigma-Aldrich (Gillingham, United Kingdom). BTEX: Benzene (B) (99%) was provided by Riedel-de Haen, (Charlotte, NC, USA). Toluene (T) (99%), ethylbenzene (E) (99%), and p-xylene (X) (99%) were obtained from Merck (Darmstadt, Germany).
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