An Agilent Infinity II MDS instrument equipped with differential refractive index (DRI), viscometry (VS), dual-angle light scatter (LS) and variable wavelength UV detectors (set at 309 nm) was used for all SEC analysis. The system was fitted with 2× PLgel Mixed D columns (300 × 7.5 mm) and a PLgel 5 μm guard column. The eluent utilized was DMF with 5 mmol NH4BF4 additive at a flow rate of 1.0 mL·min−1 at 50°C. Poly(methyl methacrylate) standards (Agilent EasyVials) were used for calibration between 955 000 and 550 g·mol−1. Samples were prepared in the mobile phase and passed through a nylon membrane with 0.22 μm pore size prior to injection. Agilent GPC/SEC Software was used to determine experimental molar mass (MnSEC), experimental molecular weight (MwSEC) and dispersity (Đ) of synthesized polymers.
Infinity 2 mds instrument
Manufactured by Agilent Technologies
The Infinity II MDS instrument is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design with interchangeable components, allowing for flexible configuration to meet specific laboratory needs. The Infinity II MDS instrument provides reliable and accurate separation, detection, and data analysis capabilities for a wide range of chemical and biological samples.
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7 protocols using infinity 2 mds instrument
1
SEC Characterization of Synthesized Polymers
2
SEC Analysis of PMMA Polymers
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SEC analysis was performed on an Infinity II MDS instrument (Agilent) equipped with differential refractive index (DRI), viscometry (VS), dual angle light scatter (LS) and variable wavelength UV detectors. The system was equipped with 2 × PLgel Mixed D columns (300 × 7.5 mm) and a PLgel 5 μm guard column. The mobile phase used was dimethylformamide (DMF) HPLC grade containing 5 mM NH4BF4 at 50 °C at flow rate of 1.0 mL min−1. Poly(methyl methacrylate) (PMMA) standards (Agilent EasyVials) were used for calibration between 555–955 000 g mol−1. Analyte samples were filtered through a nylon membrane with 0.22 μm pore size before injection. Number average molecular weights (Mn), weight average molecular weights (Mw) and dispersities (ĐM = Mw/Mn) were determined by conventional calibration using Agilent SEC software.
3
Polymer molar mass and dispersity analysis
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SEC was used for measurement
of the molar mass and dispersity of the polymers and collected on
an Agilent Infinity II MDS instrument using CHCl3 containing
2% TEA (triethylamine) as the mobile phase at 30 °C. The equipment
was fitted with a differential refractive index (DRI), dual angle
UV and viscometry detectors, 2 PLgel mixed C columns (300 mm ×
7.5 mm), and a PLgel 5 μm guard column for separation and an
autosampler for sample injection. Narrow molar mass poly(methyl methacrylate)
and polystyrene standards (Agilent EasyVials) were used for calibration
of the DRI trace. Ethanol was added to the eluent and used as a flow
rate marker. Analyte samples were filtered through a GVHP membrane
with 0.22 μm pore size before injection, and all samples were
passed through 0.2 μm PTFE filter before analysis.
of the molar mass and dispersity of the polymers and collected on
an Agilent Infinity II MDS instrument using CHCl3 containing
2% TEA (triethylamine) as the mobile phase at 30 °C. The equipment
was fitted with a differential refractive index (DRI), dual angle
UV and viscometry detectors, 2 PLgel mixed C columns (300 mm ×
7.5 mm), and a PLgel 5 μm guard column for separation and an
autosampler for sample injection. Narrow molar mass poly(methyl methacrylate)
and polystyrene standards (Agilent EasyVials) were used for calibration
of the DRI trace. Ethanol was added to the eluent and used as a flow
rate marker. Analyte samples were filtered through a GVHP membrane
with 0.22 μm pore size before injection, and all samples were
passed through 0.2 μm PTFE filter before analysis.
4
Polymer Characterization by SEC-MALS
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Size exclusion chromatography (SEC) analysis was performed on an Agilent Infinity II MDS instrument equipped with differential refractive index (DRI), viscometry (VS), dual angle light scatter (LS) and variable wavelength UV detectors. The system was equipped with 2× PLgel Mixed D columns (300 × 7.5 mm) and a PLgel 5 μm guard column. The mobile phase used was DMF (HPLC grade) containing 5 mM NH4BF4 at 50 °C at flow rate of 1.0 ml min−1. Poly(methyl methacrylate) (PMMA) standards (Agilent EasyVials) were used for calibration between 955 000–550 g moL−1. Analyte samples were filtered through a nylon membrane with 0.22 μm pore size before injection. Number average molecular weights (Mn), weight average molecular weights (Mw) and dispersities (ĐM = Mw/Mn) were determined by conventional calibration and universal calibration using Agilent GPC/SEC software.
5
SEC Characterization of Synthesized Polymers
6
Polymer Characterization by SEC-MALS
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The polymers were
measured with an Agilent Infinity II MDS instrument with differential
refractive index (DRI), viscometry (VS), dual angle light scatter
(LS), and multiple wavelength UV detectors. The system was equipped
with 2 x PLgel Mixed C columns (300 mm × 7.5 mm) and a PLgel
5 μm guard column, with CHCl3 as the eluent. Samples
were run at 1 mL/min at 30 °C. Poly(methyl methacrylate) and
polystyrene standards (Agilent EasiVials) were used for calibration.
Ethanol was added as a flow rate marker. Analyte samples were filtered
through a nylon membrane with a 0.22 μm pore size before injection.
Experimental molar mass (Mn) and dispersity
(Đ) values of the synthesized polymers were
determined by conventional calibration using Agilent GPC/SEC software.
measured with an Agilent Infinity II MDS instrument with differential
refractive index (DRI), viscometry (VS), dual angle light scatter
(LS), and multiple wavelength UV detectors. The system was equipped
with 2 x PLgel Mixed C columns (300 mm × 7.5 mm) and a PLgel
5 μm guard column, with CHCl3 as the eluent. Samples
were run at 1 mL/min at 30 °C. Poly(methyl methacrylate) and
polystyrene standards (Agilent EasiVials) were used for calibration.
Ethanol was added as a flow rate marker. Analyte samples were filtered
through a nylon membrane with a 0.22 μm pore size before injection.
Experimental molar mass (Mn) and dispersity
(Đ) values of the synthesized polymers were
determined by conventional calibration using Agilent GPC/SEC software.
7
Polymer Characterization by SEC
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Size exclusion chromatography (SEC) analysis was performed on an Agilent Infinity II MDS instrument equipped with differential refractive index (DRI), viscometry (VS), dual angle light scatter (LS) and variable wavelength UV detectors. The system was equipped with 2x PLgel Mixed C columns (300 × 7.5 mm) and a PLgel 5 µm guard column. The eluent is CHCl3 with 2% TEA (triethylamine). Samples were run at 1 mL/min at 30 °C. Poly(methyl methacrylate), and polystyrene standards (Agilent EasyVials) were used for calibration. Analyte samples were filtered through a GVHP membrane with 0.22 μm pore size before injection. Respectively, experimental molar mass (Mn, SEC) and dispersity (Đ) values of synthesized polymers were determined by conventional calibration using Agilent GPC/SEC software.
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