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Microbial media

Manufactured by HiMedia
Sourced in India

Microbial media is a solid or liquid substance used to support the growth and cultivation of microorganisms such as bacteria, fungi, and viruses. It provides the necessary nutrients, growth factors, and environmental conditions for the microorganisms to thrive. The composition and formulation of microbial media can vary depending on the specific requirements of the target microorganisms.

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2 protocols using microbial media

1

Chitosan-Based Antioxidant and Enzymatic Assays

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Chitosan (MW: ~2100 kDa and DDA: 85%, determined using gel permeation chromatography and 1H-NMR, respectively) was purchased from Marine Bio Resources Co., Ltd., Samutsakhon, Thailand. Ascorbic acid and H2O2 (50%, v/v) were acquired from Loba Chemie Pvt. Ltd., Mumbai, India. GAL, FER, CAF, CAT, and EGCG were obtained from Xi’an Julong Bio-Tech Co., Ltd. (Xi’an, China). Further, α-amylase, α-glucosidase, porcine pancreatic lipase, rat intestinal acetone powder, acarbose, orlistat, 4-nitrophenyl α-D-glucopyranoside (PNP-glycoside), and 4-methylumbelliferyl oleate (4-MU) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chemicals used for AO activities were also acquired from Sigma-Aldrich (St. Louis, MO, USA). Microbial media were bought from HiMedia Laboratories, Mumbai, India.
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2

Synthesis and Antibacterial Evaluation of Pyrrole-Based Compounds

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All chemicals used were of analytical grade used without further purification. Methyl orange (MO), ferric chloride (FeCl3), pyrrole, GA, ethylene diamine, HCl, acetone, and microbial media were purchased from Himedia, Mumbai. Gram‐positive and gram‐negative bacteria, including Staphylococcus aureus (MTCC1935) Streptococcus pneumoniae (MTCC1936), Klebsiella pneumoniae (MTCC432), Salmonella typhi (MTCC3224), Pseudomonas aeruginosa (MTCC2642), and fungi Candida albicans (MTCC3959), were purchased from Microbial Type Culture Collection, India. For aqueous solutions, deionised (DI) water was used. Experiments were conducted at room temperature (~37°C).
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