PD effluent was centrifuged (250× g, 10 min) immediately following collection, and cell-free supernatant samples were aliquoted and stored at −80 °C until further analysis. Biochemical measurements and transfer kinetics between peritoneal and systemic circulation were analyzed in serum (at 2 h of PET) and dialysate specimens by standard methods in the clinical laboratory of the Vienna General Hospital. PD effluent concentrations of creatinine were determined by a kinetic measurement of Jaffé reaction and corrected for high glucose levels by determination of a correction factor from measurements of unused PD fluid with the same method. PD effluent interleukin 6 (IL-6) and interleukin 8 (IL-8) concentrations were measured with the Immulite system (Siemens, Vienna, Austria) or by Bio-Plex bead array (Bio-Rad, Hercules, CA, USA).
Bio plex bead array
Manufactured by Bio-Rad
Sourced in Austria
The Bio-Plex bead array is a multiplex assay platform developed by Bio-Rad. It enables the simultaneous detection and quantification of multiple analytes in a single sample. The system utilizes color-coded magnetic beads coated with capture antibodies specific to the target analytes. This approach allows for the efficient, high-throughput analysis of complex samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bio plex bead array
1
Peritoneal Dialysis Effluent Analysis
2
Cytokine Quantification in Murine Aortic Tissue
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