In short, synovial tissues from 3 healthy people and 3 patients with RA were obtained to extract total RNA, from which 0.5 μg RNA was used to synthesize complementary deoxyribonucleic acid (cDNA) using a GeneChip 3′In Vitro Transcription (IVT) Express Kit (902789, Thermo Fisher Scientific Inc., Waltham, MA, USA). Then, cDNA was segmented and hybridized with human lncRNA expression array V3.0 (AS-LNC-H-V4.0, Arraystar Inc., Rockville, MD, USA). After hybridization, the array was scanned with the GeneChipTM Scanner 3000 7G system (000213, Thermo Fisher Scientific).
Genechiptm scanner 3000 7g system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The GeneChip™ Scanner 3000 7G system is a high-performance microarray scanner designed for use with Thermo Fisher Scientific's GeneChip™ microarray platform. The system is capable of scanning microarray slides and generating digital images of the hybridized arrays.
Automatically generated - may contain errors
Lab products found in correlation
As lnc h v4, by Arraystar (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Human mirna expression microarray v4, by Arraystar (1 mentions)
T4 rna ligase, by Thermo Fisher Scientific (1 mentions)
Mirna microarray chip, by Qiagen (1 mentions)
Mirvana mirna separation kit, by Thermo Fisher Scientific (1 mentions)
Genechip scanner 3000 7g, by Thermo Fisher Scientific (1 mentions)
Genechiptm wt plus reagent kit, by Thermo Fisher Scientific (1 mentions)
Genechip human gene 2.0 st array, by Thermo Fisher Scientific (1 mentions)
Genechip 3 in vitro transcription express kit, by Thermo Fisher Scientific (1 mentions)
6 protocols using genechiptm scanner 3000 7g system
1
Profiling lncRNA Expression in RA
2
miRNA Expression Profiling by Microarray
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Cells were lysed in TRIzol reagent (#15596026, Thermo Fisher Scientific) to isolate total RNA. miRNA from the total RNA sample was isolated using a mirVana miRNA separation kit (AM1561, Ambion, Austin, TX, USA). The quality and concentration of the extracted miRNA were examined using a spectrophotometer (Bio-Rad Inc.) and 1% formaldehyde-agarose gel electrophoresis. miRNA expression was determined using miRNA microarray chips (Exiqon, Vedbaek, Denmark). In short, total RNA (5 μg) was subjected to Cy3 modification at the RNA 3ʹend using the T4 RNA ligase (EL0021, Thermo Fisher Scientific). This reaction was performed at 37°C for 30 min. After that, the labeled RNA was hybridized with Human miRNA Expression Microarray V4.0 (Arraystar, Rockville, MD, USA) for 24 h. The gene expression data were obtained using a GeneChipTM Scanner 3000 7 G system (#00-0210, 2008, Thermo Fisher Scientific) and analyzed by the R Language Program (Version 3.6.3, R).
3
RNA Extraction and miRNA Profiling
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Six GC tissues were applied to extract 0.5 μg total RNA to synthesize cDNA using the GeneChip 3ʹInvitro Transcription (IVT) Express Kit (902,789, Thermo Fisher Scientific Inc., Waltham, MA, USA) as per the supplier’s instructions. Subsequently, cDNA was segmented and hybridized with human miRCURY LNA™ Universal RT microRNA PCR Human panel. After hybridization, the arrays were scanned with GeneChipTM Scanner 3000 7G system (000213, Thermo Fisher Scientific).
4
Profiling Liver Cancer Transcriptome
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In short, total RNA was isolated from cancer and paracancerous tissues from six patients with HCC. The total RNA (0.5 μg) was applied to synthesize cDNA using a GeneChip 3ʹInvitro Transcription Express Kit (902789, Thermo Fisher Scientific Inc., Waltham, MA, USA). The cDNA was then fragmented and hybridized with the human lncRNA expression array V3.0 (AS-LNC-H-V4.0, Arraystar Inc., Rockville, MD, USA). After hybridization, the microarray was washed and scanned with GeneChipTM Scanner 3000 7G system (000213, Thermo Fisher Scientific).
5
cDNA Microarray Analysis of FUT8 Silencing
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After checking RNA quality, total cDNA was synthesized and purified using GeneChipTM WT PLUS Reagent Kit (Thermo Fisher Scientific, MA, USA). Then in vitro transcription and T7 RNA amplification were performed. The fragmentation and labeling of cDNA were performed using GeneChipTM Hybridization, Wash, and Stain Kit (Thermo Fisher Scientific, MA, USA). The prepared sample was hybridized, washed and stained using an automated system (GeneChipTM Scanner 3000 7G System; Thermo Fisher Scientific, MA, USA). DNA microarray experiments were performed using GeneChip Human Gene 2.0 ST Array (Thermo Fisher Scientific, MA, USA). The hybridization signal on the chip was scanned using a GeneChip 30007G scanner (Thermo Fisher Scientific, MA, USA) and processed by microarray data analysis tool in consideration of NCBI data base, which was analyzed by a software from Filgen Inc., Nagoya, Japan. The DNA microarray expression profiles were compared between siCont (n = 3) and siFUT8#2 (n = 3).
6
Differential lncRNA Expression Analysis
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Microarray analysis was performed to screen out the differentially expressed lncRNAs between tumor and normal tissues. In brief, 0.5 µg total RNA from either tumor tissues or normal tissues was reversely transcribed to cDNA using a GeneChip 3'In vitro Transcription Express Kit (Thermo Fisher Scientific, Waltham, MA, USA). Then the cDNA was hybridized with Human LncRNA Expression Array V4.0 (Arraystar, Rockville, MD, USA), and then the chip was washed and scanned on a GeneChipTM Scanner3000 7G System (Thermo Fisher).
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