Anti rpb1 ctd 8wg16

Manufactured by Abcam

Anti-Rpb1-CTD (8WG16) is a mouse monoclonal antibody that recognizes the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpb1). This antibody is used for the detection and analysis of Rpb1-CTD in various applications.

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Lab products found in correlation

Dynabead, by Thermo Fisher Scientific (2 mentions) Sv5 pk1, by Bio-Rad (1 mentions) Anti ha probe f 7, by Santa Cruz Biotechnology (1 mentions)

2 protocols using anti rpb1 ctd 8wg16

ChIP-qPCR Protocol for Protein Interactions

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ChIP-qPCR was performed as described in Katou et al. (2003) (link) using anti-PK clone SV5-Pk1 (Serotec) and anti-Rpb1-CTD (8WG16; Abcam, ab817) coupled to Dynabeads (Invitrogen; protein A and sheep anti-mouse M280 IgG). For quantitative PCR, background controls were determined using uncoupled Dynabeads, and enrichment was normalized to chromatin input and transcription-negative site. Primers are available on request.

Poli J., Gerhold C.B., Tosi A., Hustedt N., Seeber A., Sack R., Herzog F., Pasero P., Shimada K., Hopfner K.P, & Gasser S.M. (2016). Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress. Genes & Development, 30(3), 337-354.

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ChIP-qPCR Assay for Chromatin Immunoprecipitation

Cited 1 time

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ChIP‐qPCR was performed as described in Poli et al (2016 (link)) using anti‐HA probe F‐7 (Santa Cruz, sc‐7392) and anti‐Rpb1‐CTD 8WG16 (Abcam, ab817) coupled to Dynabeads (Invitrogen, protein A and sheep anti‐mouse M280 IgG). For quantitative PCR, background controls were determined using uncoupled Dynabeads and enrichment was normalized to chromatin Input. Primers used for ChIP‐qPCR are listed in Table EV2.

Hurst V., Challa K., Jonas F., Forey R., Sack R., Seebacher J., Schmid C.D., Barkai N., Shimada K., Gasser S.M, & Poli J. (2021). A regulatory phosphorylation site on Mec1 controls chromatin occupancy of RNA polymerases during replication stress. The EMBO Journal, 40(21), e108439.

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