Rat anti tet1

Western blots were performed as described previously^{82 (link),169 (link)}. The following antibodies were used:
Rabbit anti-UHRF1 (polyclonal; 1:250; Cell Signalling, D6G8E), mouse anti-alpha-Tubulin (monoclonal; 1:500; Sigma, T9026), rabbit anti-H3 (polyclonal; 1:1000; Abcam, ab1791), mouse anti-GFP (monoclonal; 1:1000; Roche), mouse anti-FLAG M2 (monoclonal; 1:1000; Sigma, F3165), rabbit anti-xDNMT1 (polyclonal;^{82 (link)}), rabbit anti-xUHRF1 (polyclonal;^{82 (link)}), rabbit anti-USP7 (polyclonal; Bethyl Lab., A300-033A), rabbit anti-H3 (polyclonal; Abcam, ab1791), rat anti-TET1 (monoclonal; 1:10;^{176 (link)}), rat anti-alpha-Tubulin (monoclonal; 1:250; Abcam, ab6160). goat anti-rat HRP (polyclonal; 1:1000; Jackson ImmunoResearch), goat anti-rabbit HRP (polyclonal; 1:1000; BioRad), mouse anti-xCDC45 (monoclonal;¹⁷⁷ ), mouse anti-xRPA2 (monoclonal;^{178 (link)}), and mouse anti-PCNA (monoclonal; Santa Cruz, sc56). Uncropped and unprocessed scans of blots can be found in the Source Data file.

For western blot analyses, the left temporal lobe cortex tissues were first homogenized with ice-cold lysis buffer and then centrifuged at 12,000 rpm for 20 min at 4°C. Lastly, the supernatant was collected for protein content analysis. An equal amount of protein (20 ug) for each sample was loaded onto an 8 or 10% sodium dodecyl sulfate (SDS)–polyacrylamide gel and separated by electrophoresis. Then, proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore, United States). After being blocked with 5% skimmed milk for 2 h, the membrane was incubated with different primary antibodies in TBST overnight at 4°C, including rat anti-TET1 (1:1000, Abcam, United Kingdom, Cat. No. 191698), rabbit anti-TET2 (1:1000, Millipore, United States, Cat. No. ABE364) and rabbit anti-β tubulin (1:2000, Cell Signaling Technology, United States, Cat. No. 2128). After three times wash with TBST, the membrane was incubated with HRP-conjugated secondary antibody for 1 h at room temperature and probed using ECL reagent.