Avance dpx 700 nmr spectrometer

Nuclear Magnetic Resonance spectra were recorded using an Avance DPX-700 NMR spectrometer (Bruker Biospin AG, Fällanden, Switzerland) and/or an Avance DPX-500 NMR spectrometer (Bruker, Hamburg, Germany). 1H, 13C, 1D TOCSY (total correlated spectroscopy) spectra and two-dimensional (2D) spectra (correlation spectroscopy COSY, rotating frame nuclear Overhauser effect spectroscopy ROESY, nuclear Overhauser effect spectroscopy NOESY, heteronuclear single-quantum correlation spectroscopy HSQC, heteronuclear multiple-bond correlation spectroscopy HMBC) were recorded for solutions of poly- and oligosaccharides in D₂O at 35–40 °C. The concentrations of the samples ranged between 3–10 mg·mL⁻¹. Unfortunately, the structure of the HMP fraction could not be determined by NMR spectroscopy, due to insufficient spectral resolution for detailed interpretation.

Nuclear Magnetic Resonance spectra were recorded using Avance DPX-700 NMR spectrometer (Bruker Biospin AG, Fällanden, Switzerland) and Avance DPX-500 NMR spectrometer (Bruker, Hamburg, Germany). 1H, 13C, 1D TOCSY (total correlated spectroscopy) spectra and two-dimensional (2D) spectra (correlation spectroscopy COSY, rotating frame nuclear Overhauser effect spectroscopy ROESY, nuclear Overhauser effect spectroscopy NOESY, heteronuclear single-quantum correlation spectroscopy HSQC, heteronuclear multiple-bond correlation spectroscopy HMBC) were recorded for solutions of poly- and oligosaccharides in D₂O at 35–40 °C with acetone as the internal standard. The concentration of the samples was 3–10 mg/mL.