PB samples were collected through venipuncture in 2 mL BD Vacutainer® spray-coated K2EDTA blood collection tubes (BD Biosciences, San Jose, CA, USA; #367841). One hundred μL of whole blood was transported into 5 mL Corning™ Falcon™ Round-Bottom Polystyrene Tubes (#352054) and stained with 12 monoclonal antibodies against the surface markers: CD45-APC-H7 (clone 2D1, #560178); CD3-PerCP (clone SK7, #340663); CD4-BV510 (clone SK3; #562970); CD8-PE (clone HIT8a, #555635); CD14-BV605 (clone M5E2, #564054); CD16-APC (clone B73.1, #561304); CD56-APC-R700 (clone NCAM16.2, #565139); CD25-PE-CF594 (clone M-A25, #562403); CD11b-BV786 (clone D12, #742642); CD183 (CXCR3)-BV421 (clone 1C6/CXCR3, #562558); CD194 (CCR4)-BV650 (clone 1G1, #744140); and CD196 (CCR6)-BB515 (clone 11A9, #564479) (all from BD Biosciences). The staining procedure was performed in BD Horizon™ Brilliant Stain Buffer (BD Biosciences, #563794) followed by red blood cells lysis with 1x BD FACS™ lysing solution (BD Biosciences, #349202) as suggested by the manufacturer for the Lyse-no-Wash protocol.
Cd8 pe clone hit8a
Manufactured by BD
CD8-PE (clone HIT8a) is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 antigen expressed on the surface of T cells. It is a common lab reagent used for the identification and enumeration of CD8+ T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Horizon brilliant stain buffer, by BD (1 mentions)
Cd45 apc h7, by BD (1 mentions)
Cd4 bv510, by BD (1 mentions)
Falcon round bottom polystyrene tubes, by Corning (1 mentions)
Cd3 percp clone sk7, by BD (1 mentions)
Cd14 bv605, by BD (1 mentions)
Cd25 pe cf594, by BD (1 mentions)
Facs lysing solution, by BD (1 mentions)
Cd4apc clone rpa t4, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
2 protocols using cd8 pe clone hit8a
1
Comprehensive Immunophenotyping of Peripheral Blood
2
T-cell Receptor Profiling Protocol
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CD4/CD8 phenotype of TCC was determined using CD4-APC (clone RPA T4) and CD8-PE (clone HIT8a) fluorescence antibodies (BD Biosciences, Oxford, UK) by flow cytometry. Furthermore, TCC were profiled for the expression of chemokine receptors including CXCR3 (clone 49801), CCR5 (clone CTC5), CCR1 (clone 53504), CCR4 (clone 1G1), CCR9 (clone 248621), CCR8 (clone 191704), CCR6 (Clone 53103), CLA (clone HECA-452), CXCR6 (clone 56811), E-cadherin (clone 180224), CXCR1 (clone 42705), CCR2 (clone 48607), and CD69 (Clone 298614) using fluorescent antibodies purchased from R&D Systems (Minneapolis). Cells (10 000) were acquired using a FACSCanto II (BD Biosciences) and data analyzed using Cyflogic software.
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