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Nb100 544

Manufactured by Novus Biologicals
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Sourced in Japan

The NB100-544 is a laboratory equipment product manufactured by Novus Biologicals. It is a general-purpose instrument designed to perform a core function within the scientific research workflow, without further interpretation of its intended use.

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Lab products found in correlation

Ab2402, by Abcam (1 mentions) Ab2175, by Abcam (1 mentions) Ab63801, by Abcam (1 mentions) Ab79398, by Abcam (1 mentions) Ab61065, by Abcam (1 mentions) Cyclin a, by Santa Cruz Biotechnology (1 mentions) Pchk2 t68, by Cell Signaling Technology (1 mentions) Ab1791, by Abcam (1 mentions) Ppten s380, by Cell Signaling Technology (1 mentions) Brca2, by Merck Group (1 mentions) Importin β, by Abcam (1 mentions) P chk1 s317, by Cell Signaling Technology (1 mentions) Bach1, by Santa Cruz Biotechnology (1 mentions) γh2ax, by Merck Group (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) γ tubulin, by Santa Cruz Biotechnology (1 mentions) Ab180579, by Abcam (1 mentions) Sc 138, by Santa Cruz Biotechnology (1 mentions) Ab128171, by Cell Signaling Technology (1 mentions) Sc 8349, by Cell Signaling Technology (1 mentions)

2 protocols using nb100 544

1

Comprehensive Antibody Panel for DNA Damage Response

Cited 1 time
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We used the following rabbit antibodies: TOPBP1 (ab2402; Abcam), RAD51 (sc8349; Santa Cruz; ab63801; Abcam), pRAD51 S14 (Yata et al., 2012 (link)), cyclin A (NCL-CYCLINA; Leica), RPA70 (ab79398; Abcam), pRPA32 T21 (ab61065; Abcam), pRPA32 S4/8 (A300-245A; Bethyl), pRPA32 S33 (NB100-544; Novus), pCHK1 S317 (2344; Cell Signaling), pCHK2 T68 (2661; Cell Signaling), H3 (ab1791; Abcam), pH3 S10 (06–570; Millipore), BACH1 (sc-28738; Santa Cruz), pPTEN S380 (9551; Cell Signaling), pLRRK1 S1790 (H. Hanafusa, Nagoya University, Nagoya, Japan; Hanafusa et al., 2015 (link)); mouse antibodies: γH2AX (05–636; Millipore), cyclin A (sc-751; Santa Cruz), BrdU (RPN20AB; AP Biotech), RPA32 (ab2175; Abcam), BRCA1 (sc-6954; Santa Cruz), BRCA2 (OP95; Millipore), CHK1 (sc-8408; Santa Cruz), Importin-β (ab2811; Abcam), Lamin A/C (sc-7292; Santa Cruz), α-tubulin (sc-8035; Santa Cruz), PLK1 (05–844; Millipore; 331700; Zymed); and goat antibody: γ-tubulin (sc-7396; Santa Cruz).
Moudry P., Watanabe K., Wolanin K.M., Bartkova J., Wassing I.E., Watanabe S., Strauss R., Troelsgaard Pedersen R., Oestergaard V.H., Lisby M., Andújar-Sánchez M., Maya-Mendoza A., Esashi F., Lukas J, & Bartek J. (2016). TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. The Journal of Cell Biology, 212(3), 281-288.
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2

Antibody Validation for DNA Repair

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Antibodies used in this study were: Santa Cruz sc-135977 (REV7, IF, 1:100 dilution), Abcam ab180579 (REV7, IB, 1:1000 dilution), Abcam ab128171 (TRIP13, 1:1000 dilution), Cell Signaling 2144 (Tubulin, 1:5000 dilution), Novus NB100544 (pRPA32 S33, IF, 1:5000 dilution), Santa Cruz sc-8349 (RAD51, IF, 1:100 dilution), Cell Signaling 4937 (53BP1, IF, 1:1000 dilution), Bethyl A300–569A (RIF1, IF, 1:1000 dilution), Santa Cruz sc-138 (GST, 1:1000 dilution). Mitomycin C (MMC) was purchased from Sigma and Olaparib was purchased from Selleckchem.
Clairmont C.S., Sarangi P., Ponnienselvan K., Galli L.D., Csete I., Moreau L., Adelmant G., Chowdhury D., Marto J.A, & D’Andrea A.D. (2020). TRIP13 Regulates DNA Repair Pathway Choice through REV7 Conformational Change. Nature cell biology, 22(1), 87-96.
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