Glucose liquicolor

Glucose, total cholesterol, and triglyceride concentrations of the supernatant plasma from centrifuged blood were determined using Glucose LiquiColor®, LiquiColor® Triglycerides and Cholesterol LiquiColor® tests (Stanbio, Boerne, TX, USA) respectively as described in the provided protocols. Blood pressures were measured using the tail-cuff sphygmomanometer (CODA® High Throughput System, Kent Scientific Corporation, Torrington, CT, USA).

Blood samples were spun down for 10 minutes at 2000 ×g to separate the plasma. Plasma was then frozen and stored at −80°C until analysis. For the measurement of insulin (μU/ml), adiponectin (ng/ml), and leptin (ng/ml), ELISA kits were ordered from EMD Millipore (cat #s: EZHI-14K, EZHADP-61K, and EZHL-80SK, respectively) and were completed as per manufacturer's instructions. For the measurement of glucose (mg/dl), a Glucose Liquicolor (Stanbio Laboratory, REF#1070-125) reagent kit was used according to the manufacturer's protocol. The homeostatic model of insulin resistance (HOMA-IR) was used to estimate insulin resistance in these patients and was calculated as
$\begin{array}{c}\mathrm{H}\mathrm{O}\mathrm{M}\mathrm{A}‐\mathrm{I}\mathrm{R}=\frac{\mathrm{g}\mathrm{l}\mathrm{u}\mathrm{c}\mathrm{o}\mathrm{s}\mathrm{e}\left(\mathrm{m}\mathrm{g}/\mathrm{d}\mathrm{l}\right)\ast \mathrm{i}\mathrm{n}\mathrm{s}\mathrm{u}\mathrm{l}\mathrm{i}\mathrm{n}\left(\mu \mathrm{U}/\mathrm{m}\mathrm{l}\right)}{405},\end{array}$