Peroxidase affinipure goat anti rabbit igg h l secondary antibody

Manufactured by Jackson ImmunoResearch

Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) is a secondary antibody that binds to rabbit primary antibodies. It is conjugated with the enzyme peroxidase, which can be used for signal detection in various immunoassays and immunohistochemical applications.

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Lab products found in correlation

Rabbit polyclonal anti cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions) Autoradiography film, by Genesee Scientific (1 mentions) Pierce ecl substrate, by Thermo Fisher Scientific (1 mentions) Anti actin antibody a5441, by Merck Group (1 mentions)

Spelling variants of this product

Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) (57 citations) Goat anti-rabbit secondary antibody (43 citations) AffiniPure Goat Anti-Rabbit IgG (H+L) (39 citations) Goat anti-rabbit IgG (H+L) (37 citations) Peroxidase AffiniPure Goat Anti-Rabbit IgG (35 citations) AffiniPure goat anti-rabbit IgG (28 citations) Peroxidase AffiniPure Goat Anti-Rabbit IgG (H + L) antibody (3 citations) AffiniPure Goat Anti-Rabbit IgG (H + L) antibody (3 citations) Peroxidase-AffiniPure Goat Anti-Rabbit IgG antibody (3 citations) AffiniPure Goat Anti-Rabbit secondary antibody (2 citations)

3 protocols using peroxidase affinipure goat anti rabbit igg h l secondary antibody

Western Blotting of Sorted DC Lysates

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Western blotting of sorted DC lysates was performed according to a recently published protocol (13 (link)). The primary and secondary antibodies used in this assay were: Rabbit monoclonal anti-STING (D2P2F) (Cell Signaling Technology, Danvers, Massachusetts; Cat#13647), rabbit monoclonal anti-P-STING Ser³⁶⁵ (D8F4W) antibody (Cell Signaling Technology, Cat#72971), rabbit polyclonal anti-TBK1 antibody (Cell Signaling Technology, Cat#3013), rabbit monoclonal anti-TBK1 Ser¹⁷² (D52C2) antibody (Cell Signaling Technology, Cat#5483), rabbit monoclonal anti-IRF3 (D83B9) antibody (Cell Signaling Technology, Cat#4302), rabbit monoclonal anti-P-IRF3 Ser³⁹⁶ (4D4G) (Cell Signaling Technology, Cat#4947), rabbit monoclonal anti-NF-kappa-B p65 (C22B4) antibody (Cell Signaling Technology, Cat#4764), rabbit monoclonal anti-NF-kappa-B P-p65 Ser⁵³⁶ (93H1) antibody (Cell Signaling Technology, Cat#3033), rabbit polyclonal anti-cleaved caspase 3 (Asp175) antibody (Cell Signaling Technology, Cat#9661), mouse monoclonal anti-beta ACTIN, HRP (AC-15) (Abcam, Cambridge, Massachusetts; Cat#Ab49900) and Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody (Jackson ImmunoResearch Labs, West Grove, Pennsylvania, Cat#111-035-003).

Pang E.S., Daraj G., Balka K.R., De Nardo D., Macri C., Hochrein H., Masterman K.A., Tan P.S., Shoppee A., Magill Z., Jahan N., Bafit M., Zhan Y., Kile B.T., Lawlor K.E., Radford K.J., Wright M.D, & O’Keeffe M. (2022). Discordance in STING-Induced Activation and Cell Death Between Mouse and Human Dendritic Cell Populations. Frontiers in Immunology, 13, 794776.

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LPS Characterization of P. aeruginosa Strains

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Before LPS isolation, 10⁹ bacteria were pelleted from P. aeruginosa wt PA14 and PA14 waaL overnight cultures as measured by the optical density (OD 600nm). The LPS from these strains was prepared by proteinase-K digestion [31 (link)]. The bacterial LPS preparations were subjected to SDS-PAGE on 10% polyacrylamide gels. Western immunoblotting of the isolated LPS was performed using rabbit polyclonal antisera raised against the serotype O-19 strain (1:100) (generated by the Lam lab), followed by peroxidase affinipure goat anti-rabbit IgG (H+L) secondary antibody (Jackson ImmunoResearch) diluted to 1:5000 (incubated for 1h). The blot was developed using Pierce ECL substrate (Thermo Scientific) and visualized using autoradiography film (Genesee Scientific).

Demirdjian S., Schutz K., Wargo M.J., Lam J.S, & Berwin B. (2017). The Effect of Loss of O-antigen Ligase on Phagocytic Susceptibility of Motile and Non-Motile Pseudomonas aeruginosa. Molecular immunology, 92, 106-115.

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Antibodies for Protein Detection and Quantification

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Tb cryptate-labeled anti-GST antibody (anti-GST-Tb, 61GSTTLB), D2-labeled anti-FLAG M2 antibody (anti-FLAG-D2, 61FG2DLB), D2-labeled anti-GST antibody (anti-GST-D2, 61GSTDLB), Tb cryptate-labeled anti-FLAG M2 antibody (anti-FLAG-Tb, 61FG2TLB), Tb cryptate-labeled anti-His antibody (anti-His-Tb, 61HisTLB), and D2-labeled anti-His antibody (anti-His-D2, 61HisDLB) were purchased from Cisbio Bioassays. Monoclonal anti-FLAG M2-Peroxidase antibody (A8592), anti-GST-Peroxidase antibody (A7340), anti-ACTIN antibody (A5441), and anti-His antibody (SAB1306085) were purchased from Sigma. Peroxidase AffiniPure goat anti-rabbit IgG (H + L) secondary antibody (111-035-003) and Peroxidase AffiniPure goat anti-mouse IgG (H + L) secondary antibody (115-035-003) were purchased from Jackson ImmunoResearch. Anti-TMPRSS2 antibody (ab92323) was purchased from Abcam.

Cicka D., Niu Q., Qui M., Qian K., Miller E., Fan D., Mo X., Ivanov A.A., Sarafianos S.G., Du Y, & Fu H. (2023). TMPRSS2 and SARS-CoV-2 SPIKE interaction assay for uHTS. Journal of Molecular Cell Biology, 15(3), mjad017.

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