Hrp conjugated anti mouse igg

Syk inhibitors SykI and BAY 61-3606, PI3K inhibitor LY294002, ERK inhibitor U0126, JNK inhibitor SP600125, and p38 inhibitor SB203580 were purchased from Calbiochem-Merck (Darmstadt, Germany). Raf-1 inhibitor GW5074, methyl-β-cyclodextrin (MβCD), and water-soluble cholesterol were obtained from Sigma-Aldrich.
Antibodies against phospho (p)-Zap-70 (Tyr319)/Syk (Tyr352), p-Akt (Tyr308), p-c-Raf (Ser338), p-ERK1/2 (Thr202/Tyr204), p-JNK (Thr183/Tyr185), JNK, p-p38 (Thr180/Tyr182), p-IKKα/β (Ser176/180), p-NF-κBp65 (Ser536), p-IκBα (Ser32), IκBα, p-c-Jun (Ser63), c-Jun, p-c-Fos (Ser32), and c-Fos were purchased from Cell Signaling (Beverly, MA, USA). Anti-Syk, anti-β-actin, HRP-conjugated anti-rabbit IgG, and HRP-conjugated anti-mouse IgG antibodies were purchased from GeneTex Inc. (Irvine, CA, USA). Blocking antibodies against CR3 (clone 5C6) and Dectin-1 (clone 2A11) were purchased from Serotec (Oxford, UK). Antibodies for cell surface staining, anti-CD11b (clone M1/70), anti-CD18 (clone GAME-46), and APC-conjugated anti-F4/80 antibody were obtained from eBioscience (San Diego, CA, USA), and anti-Dectin-1 (clone 218820) was purchased from R&D Systems (Minneapolis, MN, USA).

Eicosane, docosane, dotriacontane, tritetracontane, heptacosane, and tetracosane were purchased from Sigma, Missouri, USA. Trypan blue solution was purchased from Glibco (Waltham, MA, USA). Cell cytotoxicity assay kit was purchased from Dojindo Molecular Technologies, INC (CK04: Cell Counting Kit-8, Tokyo, Japan). Antibodies used in the immunoblotting study includes Anti-RSV-G (Abcam, #ab94966, Cambridge, UK), β-actin (Santa Cruz, SC 4777, Dallas, TX, USA), horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (Cell signaling technology, 7074P2, Danvers, MA, USA), HRP-conjugated anti-mouse IgG (Gene Tex, GTX213111-01, Taichung, Taiwan).

Verbascoside (Acteoside) was purchased from Sigma (V4015). Trypan blue solution was purchased from Gibco (Waltham, MA, USA). Cell cytotoxicity assay kit was purchased from Dojindo Molecular Technologies, INC (CK04: Cell Counting Kit-8, Japan). Antibodies used in the immunoblotting study were as follows: Anti-RSV Glycoprotein (RSV-G) (Abcam, #ab94966, Cambridge, UK), β-actin (Santa Cruz SC 47778, Dallas, TX, USA), HRP-conjugated anti-mouse IgG (Gene Tex, GTX213111-01, Taichung, Taiwan), HRP-conjugated anti-rabbit IgG (Cell signaling technology, 7074P2, Danvers, MA, USA).