Total RNA was extracted with TRIzol reagent. Spectrophotometry (TIANGEN, OSE-260, Beijing, China) was used to detect the concentration and purity of RNA. A quantity of 1 μg total RNA was used as a template for reverse transcription PCR to synthesize complementary DNA (cDNA). Quantitative PCR (qPCR) was performed on a Step One plus PCR system (Thermo Fisher Scientific, Waltham, MA, USA) with SYBR green Premix Ex Taq (TakaRa, Kyoto, Japan). The sequences of primers are shown in Supplementary Table S1 .
Ose 260
Manufactured by Tiangen Biotech
Sourced in China
The OSE-260 is a compact and versatile laboratory instrument designed for the analysis of nucleic acids. It utilizes an optical sensing system to detect and quantify the presence of DNA or RNA in a sample. The core function of the OSE-260 is to provide accurate and reliable measurements of nucleic acid concentrations, supporting various applications in the field of molecular biology and genetics.
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2 protocols using ose 260
1
Quantitative RT-PCR Protocol for Gene Expression
2
Total RNA Extraction and cDNA Synthesis from Z. latifolia Leaves
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The total RNA of Z. latifolia leaves was extracted with the FastPure® Universal Plant Total RNA Isolation Kit (RC-401, Vazyme, Nanjing, China) according to the manufacturer’s instructions. The concentration of the extracted RNA was measured using an ultramicro spectrophotometer (OSE-260, Tiangen, Beijing, China). The extracted RNA was used as a substrate for reverse transcription using the PrimeScriptTM Ⅱ 1st Strand cDNA Synthesis Kit. The cDNA products obtained were stored at −20 °C for later use.
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