Log In Sign up
The largest database of trusted experimental protocols

Anti eomes

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Anti-Eomes is a laboratory reagent used for detection and analysis of the Eomes (Eomesodermin) protein in biological samples. Eomes is a transcription factor involved in various cellular processes. Anti-Eomes can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to identify and quantify Eomes expression.

Automatically generated - may contain errors

Lab products found in correlation

Anti nkp46, by Thermo Fisher Scientific (3 mentions) Anti gata3, by Thermo Fisher Scientific (3 mentions) Anti cd27, by BioLegend (2 mentions) Anti cxcr4, by BioLegend (2 mentions) Anti t bet, by Thermo Fisher Scientific (2 mentions) Anti α4β7, by BioLegend (2 mentions) 7 aad viability dye, by Thermo Fisher Scientific (2 mentions) Anti il 17, by BioLegend (2 mentions) Anti rat igg1k isotype control, by BioLegend (2 mentions) Anti streptavidin fluorochrome conjugates, by BioLegend (2 mentions) Anti flt3, by BioLegend (2 mentions) Anti fopx3, by Thermo Fisher Scientific (2 mentions) Anti cd196, by Thermo Fisher Scientific (2 mentions) Anti cd49b, by Thermo Fisher Scientific (2 mentions) Anti ter119, by BioLegend (2 mentions) Anti cd16 cd32, by Thermo Fisher Scientific (2 mentions) Anti cd45, by BioLegend (2 mentions) Anti cd3ε, by BioLegend (2 mentions) Anti cd19, by BioLegend (2 mentions) Anti nk1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Eomes (10 citations) Anti-Eomes (Dan11mag) (5 citations) Anti-Eomes (Dan11mag) and Live/Dead Aqua (3 citations) Rat anti-Eomes (3 citations) Fluorochrome conjugated anti-human Eomes mAb (3 citations) Alexa 488-conjugated rat anti-Eomes (2 citations) Anti-mouse Eomes (Dan11mag) (2 citations) Anti-Eomes-PE-eFlour® 610 (WD1928, (2 citations) Anti-Eomes FITC (2 citations) Anti-Eomes eFluor 660 (2 citations)

4 protocols using anti eomes

1

Comprehensive Immunostaining Panel for Immune Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-Nono (Cat# 11058-1-AP and 66361-1-Ig) was from Proteintech. Anti-Elk3 (Cat# NBP1-83960) was from Novus Biologicals. Anti-Tmem241 (Cat# 203644-T32) was from Sinobiological. Anti-Lineage cocktail (Cat# 88-7772-72), Anti-CD127 (A7R34), anti-Sca-1 (D7), anti-Flt3 (A2F10), anti-α4β7 (DATK32), anti-Id2 (ILCID2), anti-PLZF (Mags.21F7), anti-Eomes (Dan11mag), anti-NKp46 (29A1.4), anti-NK1.1 (PK136), anti-CD45 (30-F11), anti-CD25 (PC61.5), anti-Gata3 (TWAJ), anti-RORγt (AFKJS-9), anti-Bcl11b (1F8H9), anti-CD3 (17A2), anti-CD19 (1D3), anti-KLRG1 (2F1), anti-CD90 (HIS51), anti-IL-22 (IL22JOP), anti-Ki67 (SolA15), anti-CD45.2 (104), anti-BrdU (BU20A), anti-PD-1 (J43) and anti-CD45.1 (A20) were purchased from eBiosciences (San Diego, USA). Anti-c-Kit (2B8), anti-CD150 (TC15-12F12.2), anti-CD48 (HM48-1), and anti-CD49a (HMα1) were purchased from Biolegend (California, USA). Anti-β-actin (Cat# RM2001) was purchased from Beijing Ray Antibody Biotech. Paraformaldehyde (PFA) and 4’,6-diamidino-2-phenylindole (DAPI) were from Sigma. IL-22 ELISA kit was purchased from Neobioscience.
Liu N., He J., Fan D., Gu Y., Wang J., Li H., Zhu X., Du Y., Tian Y., Liu B, & Fan Z. (2022). Circular RNA circTmem241 drives group III innate lymphoid cell differentiation via initiation of Elk3 transcription. Nature Communications, 13, 4711.
+ Open protocol
+ Expand
2

Multiparametric Flow Cytometry Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell suspensions were stained with: anti-CD45 (30-F11); anti-CD45.1 (A20); anti-CD45.2 (104); anti-CD11c (N418); anti-CD11b (Mi/70); anti-CD127 (IL7Rα; A7R34); anti-CD27(LG.7F9); anti-CD8α (53-6.7); anti-CD19 (eBio1D3); anti-CXCR4(L276F12); anti-NK1.1 (PK136); anti-CD3ε (eBio500A2); anti-TER119 (TER-119); anti-Gr1 (RB6-8C5); anti-CD4 (RM4-5); anti-CD25 (PC61); anti-CD117 (c-Kit; 2B8); anti-CD90.2 (Thy1.2; 53-2.1); anti-TCRβ (H57-595); anti-TCRγδ (GL3); anti-B220 (RA3-6B2); anti-KLRG1 (2F1/KLRG1); anti-Ly-6A/E (Sca1; D7); anti-CCR9 (CW-1.2); anti-IL-17 (TC11-18H10.1); anti-rat IgG1k isotype control (RTK2071); anti-streptavidin fluorochrome conjugates from Biolegend; anti-α4β7 (DATK32); anti-Flt3 (A2F10); anti-NKp46 (29A1.4); anti-CD49b (DX5); anti-Ki67 (SolA15); anti-rat IgG2ak isotype control (eBR2a); anti-IL-22 (1H8PWSR); anti-rat IgG1k isotype control (eBRG1); anti-EOMES (Dan11mag); anti-Tbet (eBio4B10); anti-FOPX3 (FJK-16s); anti-GATA3 (TWAJ); anti-CD16/CD32 (93); 7AAD viability dye from eBiosciences; anti-CD196 (CCR6; 140706) from BD Biosciences; anti-RORγt (Q31-378) and anti-mouse IgG2ak isotype control (G155-178) from BD Pharmingen. LIVE/DEAD Fixable Aqua Dead Cell Stain Kit was purchased from Invitrogen.
Godinho-Silva C., Domingues R.G., Rendas M., Raposo B., Ribeiro H., Alves da Silva J., Vieira A., Costa R.M., Morais-Barbosa N.L., Carvalho T, & Veiga-Fernandes H. (2019). Light-entrained and brain-tuned circadian circuits regulate ILC3 and gut homeostasis. Nature, 574(7777), 254-258.
+ Open protocol
+ Expand
3

Comprehensive Multiparametric Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following monoclonal antibodies (mAbs) were purchased from BD Bioscience, BioLegend, or e-Bioscience: anti-CD4 (GK1.5), anti-CD8 (53.67), anti-CD16/32 (93), anti-CD19 (6D5), anti-CD24 (M1/19), anti-CD27(LG.3A10), anti-TCRβ (H57-597), anti-CD43 (S7), anti-CD44 (1M7), anti-CD11a (M17/4), anti-CD69 (H1.2F3), anti-NKG2D (CX5), anti-NK1.1 (PK136), anti-CD103 (2E7), anti-CCR6 (29-2L17), anti-CD122 (TM-β1), anti-CD45.1 (A20), anti-CD45.2 (104), anti-EGR2 (erongr2), anti-T-bet (eBio4B10), anti-Gata3 (TWAJ), anti-Rorγ (B2D), anti-PLZF (9E12), anti-Eomes (Dan11mag), anti-IFN-γ (XMG1.2), anti-IL-4 (11B11), anti-IL-17 (TC11-18H10), anti-IL-17RB(9B10) and CD1d-dimers. CD1d-tetramers were purchased from MBL. Fixable Aqua Dead Cell stain kit (Invitrogen) was used to eliminate dead cells. Intracellular staining for transcription factors was performed using the eBioscience Foxp3 Staining Buffer Kit. Intracellular staining for cytokines was performed using the BD Cytofix/Cytoperm™ Kit. For analysis, a FACSCalibur, Canto II or LSRFortessa X-20 instrument and the CELLQuest or FACSDiva (BD Biosciences) or FlowJo software (v10.3B2) packages were used.
Shimizu K., Sato Y., Kawamura M., Nakazato H., Watanabe T., Ohara O, & Fujii S.I. (2019). Eomes transcription factor is required for the development and differentiation of invariant NKT cells. Communications Biology, 2, 150.
+ Open protocol
+ Expand
4

Multiparametric Flow Cytometry Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell suspensions were stained with: anti-CD45 (30-F11); anti-CD45.1 (A20); anti-CD45.2 (104); anti-CD11c (N418); anti-CD11b (Mi/70); anti-CD127 (IL7Rα; A7R34); anti-CD27(LG.7F9); anti-CD8α (53-6.7); anti-CD19 (eBio1D3); anti-CXCR4(L276F12); anti-NK1.1 (PK136); anti-CD3ε (eBio500A2); anti-TER119 (TER-119); anti-Gr1 (RB6-8C5); anti-CD4 (RM4-5); anti-CD25 (PC61); anti-CD117 (c-Kit; 2B8); anti-CD90.2 (Thy1.2; 53-2.1); anti-TCRβ (H57-595); anti-TCRγδ (GL3); anti-B220 (RA3-6B2); anti-KLRG1 (2F1/KLRG1); anti-Ly-6A/E (Sca1; D7); anti-CCR9 (CW-1.2); anti-IL-17 (TC11-18H10.1); anti-rat IgG1k isotype control (RTK2071); anti-streptavidin fluorochrome conjugates from Biolegend; anti-α4β7 (DATK32); anti-Flt3 (A2F10); anti-NKp46 (29A1.4); anti-CD49b (DX5); anti-Ki67 (SolA15); anti-rat IgG2ak isotype control (eBR2a); anti-IL-22 (1H8PWSR); anti-rat IgG1k isotype control (eBRG1); anti-EOMES (Dan11mag); anti-Tbet (eBio4B10); anti-FOPX3 (FJK-16s); anti-GATA3 (TWAJ); anti-CD16/CD32 (93); 7AAD viability dye from eBiosciences; anti-CD196 (CCR6; 140706) from BD Biosciences; anti-RORγt (Q31-378) and anti-mouse IgG2ak isotype control (G155-178) from BD Pharmingen. LIVE/DEAD Fixable Aqua Dead Cell Stain Kit was purchased from Invitrogen.
Godinho-Silva C., Domingues R.G., Rendas M., Raposo B., Ribeiro H., Alves da Silva J., Vieira A., Costa R.M., Morais-Barbosa N.L., Carvalho T, & Veiga-Fernandes H. (2019). Light-entrained and brain-tuned circadian circuits regulate ILC3 and gut homeostasis. Nature, 574(7777), 254-258.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!