T7 and T3 MegaScript kits were purchased from Ambion, Life Technology (Austin, TX). LyoVec transfection reagent was purchased from Invivogen (San Diego, CA). ELISA kit for human IFN-α was purchased from PBL Assay Science (Piscataway, NJ). Rabbit antibody against TLR3 was purchased from Novus Biologicals (Littleton, CO). Rabbit antibody against GAPDH was purchased from Cell Signaling (Beverly, MA). Mouse anti-IFNAR2 antibody was purchased from LifeSpan BioScience, Inc. (Seattle, WA). Mouse IgG was purchased from Molecular Probes (Eugene, OR).
Rabbit antibody against gapdh
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit antibody against GAPDH is a laboratory reagent used for the detection and quantification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in various applications, such as Western blotting, immunohistochemistry, and ELISA. GAPDH is a commonly used housekeeping protein and this antibody can be used as a loading control in protein expression studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit antibody against gapdh
1
Transfection and Cytokine Measurement
2
Quantification of PKM2 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCT116, HepG2 and SKOV3 cells were seeded into 60 mm dish and exposed to polyplex, naked siRNA against PKM2 or transfected with Lipo 2000 (8 μL per dish) for 48 h. The cells were lysed and total protein was extracted and quantified. Protein samples were separated by 12% sodium dodecyl sulfate polacrylamine gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride (PVDF) membranes. The membranes were incubated overnight at 4 °C with rabbit antibody against PKM2 (1:1000 dilution, Cell Signaling Technology, Danvers, MA, USA) after blocking with 5% skim milk. Meanwhile, the membranes were incubated with rabbit antibody against GAPDH (1:1000 dilution; Cell Signaling Technology, Danvers, MA, USA) as an internal standard for normalization of protein levels.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!