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Goat anti mouse igg hrp sc 2055

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Goat-anti mouse IgG-HRP (SC-2055) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse immunoglobulin G (IgG) primary antibodies.

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Lab products found in correlation

Sc 47778, by Santa Cruz Biotechnology (1 mentions) Sc 7963, by Santa Cruz Biotechnology (1 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide, by Merck Group (1 mentions) Dichloromethane dcm, by Merck Group (1 mentions) Hydroxybenzotriazole, by Merck Group (1 mentions) Dodecanol, by Merck Group (1 mentions) Matrigel basement membrane matrix, by Corning (1 mentions) Goat anti rabbit igg hrp sc 2054, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Goat anti-mouse HRP (51 citations) Sc-2055 (7 citations) IgG mouse (6 citations) Mouse anti-goat (5 citations) HRP-mouse (3 citations) Mouse IgGs (3 citations) HRP-anti-goat IgG (2 citations) Mouse anti-IgG (2 citations)

2 protocols using goat anti mouse igg hrp sc 2055

1

Western Blot Analysis of Neuronal Proteins

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Nlgn3, β-catenin and β-actin proteins were analyzed by western blot using the H-55 anti-Nlgn3 antibody (SC-50395; 1:1000; Santa Cruz Biotechnology, TX, USA), the monoclonal E-5 β- catenin antibody (SC-7963; 1:2000; Santa Cruz Biotechnology) or with the anti-β-actin antibodies (SC-47778; 1:5000; Santa Cruz Biotechnology). Membranes were washed (3×) and incubated with appropriate secondary antibodies conjugated to horseradish peroxidase for 1 h at room temperature, washed and incubated for 2 min with enhanced chemiluminescence solution (Pierce ECL, Thermo Scientific, IL, USA) and exposed for 1–3 min on Carestream Kodak BioMax films (Sigma-Aldrich). Secondary antibodies were: goat-anti rabbit IgG-HRP (SC-2004 for Nlgn3, 1:5000; Santa Cruz Biotechnology), goat-anti mouse IgG-HRP (SC-2055, for β- catenin and β-actin; 1:5000; Santa Cruz Biotechnology).
Medina M.A., Andrade V.M., Caracci M.O., Avila M.E., Verdugo D.A., Vargas M.F., Ugarte G.D., Reyes A.E., Opazo C, & De Ferrari G.V. (2018). Wnt/β-catenin signaling stimulates the expression and synaptic clustering of the autism-associated Neuroligin 3 gene. Translational Psychiatry, 8, 45.
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2

Cardiomyocyte Differentiation Assay

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CYP was purchased from Logan Natural Product (Plano, TX), and DTX was obtained from LC Laboratories (Woburn, MA). Dodecanol (DC), 1-ethyl-3-(3-(dimethylamino)propyl carbodiimide, N,N′-dicyclohexylcarbodiimide (EDC), N,N-dimethylpyridin-4-amine (DMAP), N,N′-Dicyclohexylcarbodiimide (DCC), Boc-β-alanine, dichloromethane (DCM), and hydroxybenzotriazole (HOBT) were obtained from Sigma-Aldrich (St. Louis, MO). Primary antibodies Shh (rabbit polyclonal antibody sc-9024), Bax (rabbit polyclonal antibody sc-6236) and GAPDH (mouse monoclonal antibody sc-365062) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Secondary antibodies goat anti-rabbit IgG-HRP (sc-2054) and goat anti-mouse IgG-HRP (sc-2055) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Matrigel® matrix basement membrane was obtained from Corning (Chicago, IL). All other chemicals were analytical grade and were purchased from Sigma-Aldrich (St. Louis, MO).
Almawash S.A., Mondal G, & Mahato R.I. (2018). Coadministration of Polymeric Conjugates of Docetaxel and Cyclopamine Synergistically Inhibits Orthotopic Pancreatic Cancer Growth and Metastasis. Pharmaceutical research, 35(1), 17.
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