Superose 3.2 300 column

Manufactured by GE Healthcare

The Superose 3.2/300 column is a size-exclusion chromatography (SEC) column designed for the separation and analysis of macromolecules such as proteins, peptides, and other biological molecules. It features a packed bed of Superose resin, which provides efficient separation and high resolution. The column has a flow path of 3.2 mm in diameter and a bed length of 300 mm, making it suitable for use in analytical and semi-preparative applications.

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Lab products found in correlation

Superdex 10 300 column, by GE Healthcare (1 mentions)

Spelling variants of this product

Superose 6 Increase 3.2/300 column (26 citations) Superose 6 3.2/300 column (16 citations) Superose 6 PC 3.2/300 column (4 citations) Superose 6 Increase 3.2/300 size-exclusion chromatography column (2 citations) Superose 6 Increase 3.2/300 GL column (2 citations) Superose 6 increase 3.2/300 mm column (2 citations) Superose 6 3.2/300 gel filtration column (2 citations) Superose 6 increase 3.2/300 size exclusion column (2 citations) Superose 200 Increase 3.2/300 GL column (1 citations)

2 protocols using superose 3.2 300 column

SEC Analysis of TAp63α Isoform

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Analytical SEC was performed in phosphate buffer (50 mM sodium phosphate pH 7.8,
100 mM NaCl) at 4°C using a Superose 3.2/300 column (GE Healthcare) (injection volume
50 μL; flow rate 50 μL/min; fraction size 50 μL). SEC fractions were quantified by
western blotting. Analytical SEC of TAp63α_min in urea was performed as
described detailed in Supplemental Experimental Procedures.

Coutandin D., Osterburg C., Srivastav R.K., Sumyk M., Kehrloesser S., Gebel J., Tuppi M., Hannewald J., Schäfer B., Salah E., Mathea S., Müller-Kuller U., Doutch J., Grez M., Knapp S, & Dötsch V. (2016). Quality control in oocytes by p63 is based on a spring-loaded activation mechanism on the molecular and cellular level. eLife, 5, e13909.

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Size-Exclusion Chromatography of Protein Complexes

Cited 1 time

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Analytical SEC was performed in phosphate buffer (50 mM Tris pH 7.5, 150 mM NaCl, 20 mM CHAPS, 1 mM DTT) at 4 °C using a Superose 3.2/300 column (GE Healthcare) (injection volume 50 μl; flow rate 50 μl/min; fraction size 50 μl). SEC fractions were quantified by western blotting. Analytical SEC of TDs was performed in KPKCl buffer at 4 °C using a Superdex 10/300 column (GE Healthcare) [32 (link)].

Raab M., Matthess Y., Raab C.A., Gutfreund N., Dötsch V., Becker S., Sanhaji M, & Strebhardt K. (2021). A dimerization-dependent mechanism regulates enzymatic activation and nuclear entry of PLK1. Oncogene, 41(3), 372-386.

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