FACS was used to demonstrate the transfer of RNA from exosomes into recipient cells. This was achieved by exposing C33A cells to Hela exosomes (20μl) containing either normal RNA or BrUTP-labelled RNA for 24 or 48 hours. The exosome exposed cells were collected, permeabilised and stained with an anti-BrdU primary antibody (B8434) and an IgG-FITC secondary antibody (F0257) (Sigma-Aldrich). The C33A exosome exposed cells were analysed with a BD Biosciences FACSCanto II flow cytometer at the Biological Resources Imaging Laboratory at UNSW as a fee for service.
Anti brdu primary antibody b8434
Manufactured by Merck Group
The Anti-BrdU primary antibody (B8434) is a monoclonal antibody that specifically recognizes bromodeoxyuridine (BrdU), a synthetic nucleoside commonly used to label proliferating cells. This antibody can be used to detect and quantify BrdU incorporation in various cell types and experimental applications.
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Lab products found in correlation
2 protocols using anti brdu primary antibody b8434
1
Exosome-Mediated RNA Transfer Assay
2
Exosome RNA Transfer Visualization
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FACS was used to demonstrate the transfer of RNA from exosomes into recipient cells. This was achieved by exposing C33A cells to Hela exosomes (20 μl) containing either normal RNA or BrUTP-labelled RNA for 24 or 48 h. The exosome exposed cells were collected, permeabilised and stained with an anti-BrdU primary antibody (B8434) and an IgG-FITC secondary antibody (F0257) (Sigma–Aldrich). The C33A exosome exposed cells were analysed with a BD Biosciences FACSCanto II flow cytometer at the Biological Resources Imaging Laboratory at UNSW as a fee for service.
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