Filter plate

Manufactured by PerkinElmer

The Filter Plate is a laboratory equipment designed to facilitate the filtration and separation of samples. It features a multi-well format that allows simultaneous processing of multiple samples. The core function of the Filter Plate is to provide a platform for efficient liquid filtration and sample preparation workflows.

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Lab products found in correlation

Pipetting robot, by Beckman Coulter (3 mentions) 96 well plate, by BD (3 mentions) Decorin, by Merck Group (3 mentions) Topcount nxt, by PerkinElmer (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Scintillation fluid, by PerkinElmer (1 mentions)

3 protocols using filter plate

Parasite Binding Assay with Decorin

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Example 13

Parasite DNA was labeled with Tritium by overnight incorporation of titrated hypoxanthine. A 96 well plate (Falcon) was coated with 2 μg/ml of Decorin (Sigma-Aldrich) overnight and blocked with 2% bovine serum albumin (Sigma) as described (Nielsen et al., 2009). Tritium labeled late-stage IEs were MACS purified and added to the 96 well plate in a concentration of 200,000 cells per well. Titrations of serum were added in a total volume of 100 μl in triplicate wells. After incubation for 90 min at 37° C., unbound IEs were washed away by a pipetting robot (Beckman-Coulter). The remaining IEs were harvested onto a filter plate (Perkin-Elmer). After addition of scintillation fluid (Perkin-Elmer) the counts per minute (CPM) recording the number of non-inhibited IE was determined by liquid scintillation counting on a Topcount NXT (Perkin-Elmer). Data were adjusted to percentage of binding by dividing test result with the mean value of wells with IE incubated without serum.

US11129882B2. Virus like particle with efficient epitope display (2021-09-28). University of Copenhagen [DK]. Inventors: Adam Frederik Sander Bertelsen [DK], Ali Salanti [DK], Thor Theander [DK], Susan Thrane [DK], Christoph Mikkel Janitzek [DK], Mette Ørskov [DK], Morten Agertoug Nielsen [DK].

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Decorin-based IE Binding Assay

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Parasite DNA was labeled with Tritium by overnight incorporation of titrated hypoxanthine. A 96 well plate (Falcon) was coated with 2 μg/ml of Decorin (Sigma-Aldrich) overnight and blocked with 2% bovine serum albumin (Sigma) as described [23 (link)]. Tritium labeled late-stage IEs were MACS purified and added to the 96 well plate in a concentration of 200,000 cells per well. Titrations of serum were added in a total volume of 100 μl in triplicate wells. After incubation for 90 min at 37°C, unbound IEs were washed away by a pipetting robot (Beckman-Coulter). The remaining IEs were harvested onto a filter plate (Perkin-Elmer). After addition of scintillation fluid (Perkin-Elmer) the counts per minute (CPM) recording the number of non-inhibited IE was determined by liquid scintillation counting on a Topcount NXT (Perkin-Elmer). Data were adjusted to percentage of binding by dividing test result with the mean value of wells with IE incubated without serum.

Thrane S., Janitzek C.M., Agerbæk M.Ø., Ditlev S.B., Resende M., Nielsen M.A., Theander T.G., Salanti A, & Sander A.F. (2015). A Novel Virus-Like Particle Based Vaccine Platform Displaying the Placental Malaria Antigen VAR2CSA. PLoS ONE, 10(11), e0143071.

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Malaria Parasite Binding Assay

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Plasmodium falciparum (FCR3 genotype) parasites were maintained in culture as described [60 (link)]. Parasites were panned on BeWo cells to select for a chondroitin sulphate A (CSA)-binding phenotype, as described [61 (link)]. Parasite DNA was labeled with Tritium by overnight incorporation of titrated hypoxanthine. A 96-well plate (Falcon) was coated with 2 μg/ml of Decorin (Sigma-Aldrich) overnight and blocked with 2 % bovine serum albumin (Sigma) as described [60 (link)]. Tritium labeled late-stage infected erythrocytes (IEs) were MACS purified and added to the 96-well plate in a concentration of 200,000 cells per well. Titrations of serum were added in a total volume of 100 μl in triplicate wells. After incubation for 90 min at 37 °C, unbound IEs were washed away by a pipetting robot (Beckman-Coulter). The remaining IEs were harvested onto a filter plate (Perkin-Elmer). After addition of scintillation fluid (Perkin-Elmer) the counts per minute (CPM) recording the number of non-inhibited IE was determined by liquid scintillation counting on a Topcount NXT (Perkin-Elmer). Data were adjusted to percentage of binding by dividing test result with the mean value of wells with IE incubated without serum.

Thrane S., Janitzek C.M., Matondo S., Resende M., Gustavsson T., de Jongh W.A., Clemmensen S., Roeffen W., van de Vegte‑Bolmer M., van Gemert G.J., Sauerwein R., Schiller J.T., Nielsen M.A., Theander T.G., Salanti A, & Sander A.F. (2016). Bacterial superglue enables easy development of efficient virus-like particle based vaccines. Journal of Nanobiotechnology, 14, 30.

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