Anti cd93

Manufactured by BioLegend

Sourced in United States

Anti-CD93 is a monoclonal antibody that binds to the CD93 antigen. CD93 is a transmembrane glycoprotein involved in cell-cell interactions and phagocytosis. This antibody can be used for the identification and study of cells expressing CD93.

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Anti-CD93-PE (2 citations)

3 protocols using anti cd93

Comprehensive Immunophenotyping Panel

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The following antibodies were purchased from Biolegend Inc. (San Diego, CA, USA): anti-CD3-pacific blue (17A2), anti-CD4-pacific blue (GK1.5), anti-NK1.1-pacific blue (PK136), anti-CD11b-pacific blue (M1/70), anti-CD11C-pacific blue (N418), anti-Ter-119-pacific blue (Ter-119), anti-CD8-pacific blue (53-6.7), anti-B220-pacific blue (RA3-61B2), anti-CD4-PE (GK1.5), anti-CD4-FITC (GK1.5), anti-CD8-APC (53-6.7), anti-CD8-Pecy7 (53-6.7), anti-CD23-PECy7 (B3B4), anti-CD21/CD35 (CR2/CR)-PerCP/Cy5.5 (7E9), anti-Gr1-Pecy7 (RB6-8C5), Anti-IAb-FITC (KH74), anti-B220-APC-Cy7 (RA3-61B2), anti-CD117-PE-Cy7 (2B8), anti-PDCA1-APC (927), anti-CD135 (flt3)-APC (A2F10), anti-CD25-PE (3C7), anti-CD11C (N418), anti-IgD-PerCP/Cy5.5 (11-26c.2a), anti-Sca1-APC (D7), anti-IgM-PE (RMM-1), anti-CD16/32-APC/Cy7(93), anti-CD127 (IL-7R)-PerCP/Cy5.5 (SB/199), anti-CD93 (AA4.1)-PE (AA4.1), anti-CD117 (c-kit)-PE/Cy7 (2B8), and anti-CD34-PE (MEC14.7). Anti-human/mouse phospho-ERK1/2 (T202/Y204)-APC (MILAN8R), anti-human-mouse phospho-p38 (T180/Y182)-APC (4NIT4KK), and anti-human/mouse phosphor-AKT (S473)-APC (SDRNR) were purchased from eBioscience (San Diego, CA, USA).

Yang Y., Xu J., Chen H., Fei X., Tang Y., Yan Y., Zhang H, & Zhang J. (2016). MiR-128-2 inhibits common lymphoid progenitors from developing into progenitor B cells. Oncotarget, 7(14), 17520-17531.

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Stimulation and Flow Cytometric Analysis of Splenocytes

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Splenocytes (2 × 10⁶ cells/well) were stimulated with 10 μg/mL anti-IgM (31178, Invitrogen) or 10 μg/mL anti-IgD Ab (2057001, Invitrogen) for 4, 14, and/or 24 hours. Splenocytes were stained with fluorescent conjugated anti-CD25 (PC61.5, Tonbo Biosciences), anti-CD69 (H1.2F3, BioLegend), and anti-B220 (RA3.6B2, BioLegend) or anti-B220 (RA3-6B2, BioLegend), anti-CD93 (AA4.1 BioLegend), anti-CD21 (7E9, BioLegend), and anti-CD23 (B3B4, BioLegend), and they were analyzed by flow cytometry.

Avalos A., Tietsort J.T., Suwankitwat N., Woods J.D., Jackson S.W., Christodoulou A., Morrill C., Liggitt H.D., Zhu C., Li Q.Z., Bui K.K., Park H, & Iritani B.M. (2022). Hem-1 regulates protective humoral immunity and limits autoantibody production in a B cell–specific manner. JCI Insight, 7(9), e153597.

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Characterization of Murine B Cell Populations

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Lymphocytes were isolated from the spleen and BM of mice.
The following antibodies were used for flow cytometry: anti-B220, anti-CD93, anti-CD21, anti-CD23, anti-Bcl-2, (BioLegend), anti-Igj, anti-Igk-1, anti-Igk-2 and anti-Igk-3 (BD Biosciences, Franklin Lakes, NJ, USA). Stained cells were analyzed on a FACSCanto flow cytometer (BD Biosciences). Cytoplasmic immunoglobulins were stained using the Cytofix/Cytoperm Kits (BD Biosciences) according to the manufacturer's instruction. Bcell survival was determined using the Fixation and Dead Cell Discrimination Kit (Miltenyi Biotec) or 7-amino-actinomycin D (7AAD) (BD Biosciences) staining and the Caspase-3 detection kit (FITC-DEVD-FMK) (Merck, Kenilworth, NJ, USA) according to the manufacturer's instructions.

Shinohara H., Nagashima T., Cascalho M.I, & Kurosaki T. (2016). TAK1 maintains the survival of immunoglobulin λ-chain-positive B cells. Genes to cells : devoted to molecular & cellular mechanisms, 21(11).

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