Thinprep 2000 system

Manufactured by Hologic

Sourced in United States

The ThinPrep 2000 System is a fully automated laboratory instrument designed for the preparation of cell samples for cytological examination. The system processes liquid-based cytology samples, such as those collected for cervical cancer screening, and produces a thin, evenly dispersed cell sample on a glass slide for microscopic analysis.

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Lab products found in correlation

Cytolyt, by Hologic (1 mentions) Cytobrush, by Qiagen (1 mentions) Thinprep cytologic test, by Hologic (1 mentions) Preservcyt solution, by Hologic (1 mentions)

8 protocols using thinprep 2000 system

LBC Slide Preparation and Reporting

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LBC slide preparation was carried out using the ThinPrep-2000 system (Hologic) automated slide preparation unit, according to the manufactures directions and Pap stained (Hologic) at the Regional Cytology Laboratories (Clinical Sciences Building, Manchester Royal Infirmary, UK). Reporting of the LBC samples was done using a Dual Review Imaging System (Hologic).

Hampson L., Maranga I.O., Masinde M.S., Oliver A.W., Batman G., He X., Desai M., Okemwa P.M., Stringfellow H., Martin-Hirsch P., Mwaniki A.M., Gichangi P, & Hampson I.N. (2016). A Single-Arm, Proof-Of-Concept Trial of Lopimune (Lopinavir/Ritonavir) as a Treatment for HPV-Related Pre-Invasive Cervical Disease. PLoS ONE, 11(1), e0147917.

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Automated Liquid-Based Cervical Cytology

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The LBC test was performed using an automated liquid-based monolayer cell preparation system (ThinPrep 2000 system; Hologic, Inc., Marlborough, MA, USA). The samples were immersed in Cytolyt (Hologic, Inc., Marlborough, MA, USA) and transferred to a PreservCyt bowl. The cylinder with a filtration membrane was then placed in PreservCyt bowl and was rotated to ensure that the cells were homogeneously distributed. When a vacuum was applied, the erythrocytes and mucus were removed by negative pressure. Thus, only the cells left on the filtration membrane were attached to the slide and then fixed in 95% ethyl alcohol at room temperature. Following fixation for 30 min, smears of specimens were cytologically stained using standard Papanicolaou staining as previously described (25 (link)). All slides were evaluated routinely by an experienced cytopathologist without any prior knowledge of the immunohistochemical findings.

Xu X., Li P., Fu D., Wei Z., Xu S., Xu F., Tian F., Ge J., Zhang Z, & Cheng W. (2018). Combined use of urinary Survivin detection and liquid-based cytology for the early diagnosis of bladder urothelial carcinoma. Oncology Letters, 15(5), 7739-7743.

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Liquid-based Cervical Cytology and HPV Testing

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Cervical cytological samples were collected in ThinPrep fixative (Hologic, Marlborough, MA, USA) for the liquid‐based Pap test.²⁹ ThinPrep slides were prepared, stained and processed by using the ThinPrep 2000 System (Hologic). According to TBS 2001, the diagnostic terms on Pap smears were classified into categories as follows: NILM, ASC‐US, LSIL, ASC‐H, HSIL and SCC. All Pap slides were independently analysed by two pathologists. Discrepant diagnoses were reviewed for consensus. The remnant cervical sample of each woman after the preparation of a liquid‐based Pap slide was further used for p16^INK4A FCM (10 mL) and HPV DNA testing (5 mL), that is, approximately 15 mL in total.

He Y., Shi J., Zhao H., Wang Y., Zhang C., Han S., He Q., Li X., Li S., Wang W., Yi M., Hu X., Xing Z., Han H., Gao Y., Zhou Q., Lu L., Guo J., Cao H., Lu C., Hou Y., Chen D., Yang F., Lei P., Di W., Qian J., Xia Y., Zhang Y., Deng Y., Zhu J, & Xu C. (2023). p16INK4A flow cytometry of exfoliated cervical cells: Its role in quantitative pathology and clinical diagnosis of squamous intraepithelial lesions. Clinical and Translational Medicine, 13(3), e1209.

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Thin-layer Cytology for Cervical Cancer Screening

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Thin-layer cytology slides were prepared with the ThinPrep 2000 System (Hologic, Boxborough, MA). The preparation process adopted the common gynecology sample procedure: the cell suspension was firstly homogenized after the PreservCyt container placed in the instrument and then cells were collected through a filter membrane to a glass slide. Cells were fixed with 95% ethanol and stained using the Papanicolaou method. Cytology slides were interpreted by two experienced cytotechnologists and confirmed by a third pathologist using the classification of the 2001 Bethesda reporting system. Positive cytology results were defined as atypical squamous cells of undetermined significance or worse, which led to referral to colposcopy.

Wang H.R., Li Y.C., Guo H.Q., Yu L.L., Wu Z., Yin J., Liao G.D., Qu Y.M., Jiang Y., Wang D, & Chen W. (2017). A cocktail of p16INK4a and Ki-67, p16INK4a and minichromosome maintenance protein 2 as triage tests for human papillomavirus primary cervical cancer screening. Oncotarget, 8(48), 83890-83899.

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Cervical Specimen Collection and Cytology Evaluation

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The present study was approved by the Ethics Committee of the School of Medicine, Jinan University (Guangzhou, China). Cervical specimens were collected with a Cytobrush (Qiagen, Inc., Valencia, CA, USA) and placed into a ThinPrep Pap test vial (Hologic, Inc., Marlborough, MA, USA) containing 20 ml PreservCyt Solution (Hologic, Inc.). Referral Pap specimens were used for the ThinPrep cytologic test, which was performed by Kingmed Center for Clinical Laboratory Corporation (Guangzhou, China) using the ThinPrep 2000 System (Hologic, Inc.) and evaluated for routine screening cytology. A Pap smear was positive for a squamous intraepithelial lesion (SIL) if there was a low (LSIL) or high (HSIL) grade SIL, as classified according to the Bethesda Classification System (16 (link)). Excluding cases with other types of uterine disease and without a history of hysterectomy, a total of 164 cases [120 LSIL (73.2%) and 44 HSIL (26.8%)] were recruited. A total of 296 control samples without an intraepithelial lesion or malignancy were recruited from the area of residence of the cases. The study was explained to each individual and written consent for their participation was obtained.

Ye X., Jing L., Zhong X., Xiao D., Ou M., Guo C., Yang G., Jing C, & Wei X. (2017). Interactions between polymorphisms in the 3′untranslated region of the cyclin dependent kinase 6 gene and the human papillomavirus infection, and risk of cervical precancerous lesions. Biomedical Reports, 6(6), 640-648.

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Cervical Specimen Collection and Cytology

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The study was approved by the Ethics Committee of School of Medicine in Jinan University. Cervical specimens were collected with a broom-like device (Qiagen, Valencia) and placed into a ThinPrep Pap test vial containing PreservCyt Solution. Referral Pap specimens were processed locally using the ThinPrep 2000 System (Hologic) and evaluated for routine screening cytology. A pap smear was positive for SIL if low (LSIL) and high (HSIL) grade squamous intraepithelial lesion, as classified according to the Bethesda Classification System, was detected [41 (link)]. Excluding other types of uterine diseases and no history of hysterectomy, a total of 164 cases were composed of 120 LSIL (73.2%) and 44 HSIL (26.8%). A total of 428 control samples without intraepithelial lesion or malignancy were recruited from the area of residence of the cases.

Xiao D., Huang W., Ou M., Guo C., Ye X., Liu Y., Wang M., Zhang B., Zhang N., Huang S., Zang J., Zhou Z., Wen Z., Zeng C., Wu C., Huang C., Wei X., Yang G, & Jing C. (2016). Interaction between susceptibility loci in cGAS-STING pathway, MHC gene and HPV infection on the risk of cervical precancerous lesions in Chinese population. Oncotarget, 7(51), 84228-84238.

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Cervical Sample Collection and Cytologic Analysis

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Cervical samples were collected using a disposable cervical brush (Ningbo HLS Medical Products Co., Ltd.) after menstruation, and then the samples were stored and transported in PreservCyt solution for thinprep cytologic test (TCT) using ThinPrep 2000 system (Hologic Inc.). The results were classified into 7 groups: negative for intraepithelial lesion or malignancy (NILM), atypical squamous cells of undetermined significance (ASC‐US), low‐grade squamous intraepithelial lesions (LSIL), atypical squamous cells‐cannot exclude high‐grade squamous intraepithelial lesions (ASC‐H), high‐grade squamous intraepithelial lesions (HSIL), atypical glandular cells (AGC), and carcinoma (including squamous cell carcinoma and adenocarcinoma) based on the Bethesda System of 2001,22 while unsatisfactory results were excluded.

Wang Y., Xue J., Dai X., Chen L., Li J., Wu Y, & Hu Y. (2018). Distribution and role of high‐risk human papillomavirus genotypes in women with cervical intraepithelial neoplasia: A retrospective analysis from Wenzhou, southeast China. Cancer Medicine, 7(7), 3492-3500.

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Liquid-based Cervical Cytology Preparation

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For liquid-based cytological smears, samples were taken from the fornix, portio, and endocervix. The ThinPrep test (Hologic Inc., Marlborough, MA, USA) was performed using an automated liquid-based monolayer cell preparation system (ThinPrep 2000 system; Hologic Inc.), according to manufacturer's recommendations. Briefly, the samples were immersed in CytoLyt buffer (Hologic Inc.) and then transferred to a PreservCyt bowl (Hologic Inc.). Cells were released by pushing the brush to the bottom, forcing the bristles apart, and swirling the brush in the fluid. A cylinder containing a filtration membrane was placed in the bowl and rotated to ensure homogeneous cell distribution. Erythrocytes and mucus were allowed to penetrate the filtration membrane under negative pressure, leaving the cell membranes on the filtration membrane. Each ThinPrep slide was fixed in ethanol and stained using the Papanicolaou method. Tumor diathesis, cellular composition, and cellular arrangement of the specimens were evaluated.

Jung Y.Y., Nahm J.H, & Kim H.S. (2016). Cytomorphological characteristics of glassy cell carcinoma of the uterine cervix: histopathological correlation and human papillomavirus genotyping. Oncotarget, 7(45), 74152-74161.

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