Dasatinib

Cryptotanshinone (CPT) was purchased from Shanghai Yuanye Biotechnology Co., Ltd, with a purity of 98% (#B21586, Shanghai, China). High-performance liquid chromatography (HPLC) analysis was applied to further confirm the purity of CPT (98%). Imatinib (Genike) was obtained from Nanjing Chia Tai Tianqing Pharmaceutical Co., Ltd. The second-generation tyrosine kinase inhibitors nilotinib was purchased from Aladdin Chemistry Co., Ltd (#N126111, Shanghai, China). Dasatinib was obtained from Shanghai Yuanye Biotechnology Co., Ltd (#S45672, Shanghai, China). Antibodies used in this study were purchased from Cell Signalling Technology (MA, USA), including cleaved caspase-3 (#9664), cleaved PARP (#5625), p-STAT3 (#9145), Bcr-Abl (#3908), p-Bcr-Abl (#3009), p-Src (#2101) and β-Actin (#3700). Antibody for cleaved caspase-9 (Asp353) (#AF5240) was obtained from Affinity Biosciences (Cincinnati, OH, USA), for eIF4E (#610270) was obtained from BD Biosciences (San Diego, CA, USA), and p-eIF4E (ab76256) was purchased from Abcam. The rabbit polyclonal antibody against Ki-67 (PB9026) and mouse monoclonal antibody against PCNA (BM0104) was obtained from Boster Biological Technology Co., Ltd.

The following antibodies were used in this study: rabbit anti-Myo19 (HPA059715, 1:1000 for western blotting and 1:800 for IHC) and mouse anti-α-tubulin (T9026, 1:4000 for western blotting) from Sigma-Aldrich; rabbit anti-Mic60 (A2751, 1:1000 for western blotting), rabbit anti-Src (A19119, 1:1000 for western blotting) rabbit anti- pSrc Y416 (AP0452, 1:1000 for western blotting) from ABclonal; mouse anti-RhoA (ARH04, 1:800 for western blotting) from Cytoskeleton; rabbit anti-GAPDH (ab181602, 1:4000 for western blotting) from Abcam; mouse anti-Nrf2 (sc-365949, 1:800 for western blotting) from Santa Cruz; anti-mouse (sc-516102, 1:4000) and anti-rabbit (sc-2004, 1:4000) horseradish peroxidase (HRP)-conjugated secondary antibodies from Santa Cruz Biotechnology.
For reagents, CellTracker™ Red CMTPX (C34552), CellTracker™ Green CMFDA (C2925) and MitoSOX (M36008) were purchased from ThermoFisher Scientific. 2-Deoxy-D-glucose (HY-13966) was purchased from MedChemExpress. H₂O₂ (H112515), catalase (C163049), and MitoPY (M286913) were purchased from Aladdin. PP2 (SC1234) was purchased from Beyotime. Rho activator II (CN03) was purchased from Cytoskeleton. Dasatinib (S45672) was purchased from Yuanye. MitoTEMPO (S9733) was purchased from Selleck. Ehop-016 (BD559120) was purchased from Bidepharm. AntimycinA (ab141904) was purchased from Abcam.