Ef s18 55 is lens

Mice were randomized to 4 groups and treated as follows: (G₁) saline (vehicle/control, ip, weekly, 4 weeks); (G₂) GEM (80 mg/kg, ip, weekly, 4 weeks); (G₃) BEV (5 mg/kg, ip, twice a week, 4 weeks) / GEM (Eli Lilly, Indianapolis, IN) (80 mg/kg, ip, weekly, 4 weeks) and (G₄) BEV (Genentech, South San Francisco, CA) (5 mg/kg, ip, twice a week, 2 weeks) / GEM (80 mg/kg, ip, weekly, 2 weeks) → S. typhimurium A1-R (5 × 10⁷ CFU/body, iv, weekly, 2 weeks) (Fig. 4A). Each treatment group comprised 5 tumor-bearing mice. Body weights of the mice were determined on a balance once a week. Tumor size was measured with calipers in the subcutaneous models and at laparotomy in the orthotopic models on day 36. Tumors were imaged with an OV100 Small Animal Imaging System (Olympus, Tokyo, Japan) or a Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon, Tokyo, Japan) and weighed and harvested for analysis.

For fluorescence-guided surgery (FGS), a 15-mm transverse incision was made on the left flank of the mouse through the skin and peritoneum which was kept open with a retractor. The tail of the pancreas was exposed through this incision. Fifty µg of anti-CA19-9 antibody, conjugated to DyLight 650, was injected via the tail vein in the mice in the FGS group 24 hours before surgery. A MINI MAGLITE LED PRO flashlight (MAG INSTRUMENT, Ontario, CA, USA) coupled to an excitation filter (ET 640/30X, Chroma Technology Corporation, Bellows Falls, VT, USA) was used as the excitation light source. A Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon, Tokyo, Japan) coupled with an emission filter (HQ700/75M-HCAR, Chroma Technology Corporation) was used as the real-time image capturing device for FGS. BLS was performed under standard bright-field using an MVX10 microscope (Olympus, Tokyo, Japan). After completion of surgery, the incision was closed in one layer using 6-0 nylon surgical sutures, and the mice were allowed to recover in their cages.