RNA was extracted and purified from cultured and uncultured cells using the ReliaPrep RNA Cell Miniprep System (Promega, Madison, WI, USA). Total RNA was converted to cDNA by reverse transcription using the Revert Aid Kit (Thermo Fisher Scientific, St. Leon-Rot, Germany). Quantitative real-time polymerase chain reaction was performed using the GoTaq qPCR Master Mix with BRYTE green (Promega). A 7500 real-time PCR system was used and data was analyzed using System SDS software v2.0.6 (Applied Biosystems). Fold change differences between samples were determined using the comparative Ct method (∆∆Ct). The expression level of different target genes, relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the calibrator, was given by 2-∆∆Ct. Gene-specific primers were produced by Integrated DNA Technologies (Leuven, Belgium).
System sds software v2
Manufactured by Thermo Fisher Scientific
System SDS software v2.0.6 is a software application designed for managing and accessing safety data sheets (SDS) related to laboratory equipment and chemicals. The core function of this software is to provide a centralized platform for storing, retrieving, and managing SDS information.
Automatically generated - may contain errors
2 protocols using system sds software v2
1
Quantitative Real-Time PCR Analysis
2
Total RNA Extraction and qPCR Analysis
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Total cell RNA was extracted and purified using the Reliaprep RNA cell Miniprep System (Promega, Madison, WI) and converted to cDNA by reverse transcription using the Revert Aid Kit (ThermoFisher Scientific, St. Leon-Rot, Germany). Quantitative real-time polymerase chain reaction was performed using the GoTaq qPCR Master Mix with BRYTE green (Promega). A 7500 real time PCR system was used and data was analyzed using System SDS software v2.0.6 (Applied Biosystems).
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