Percentage diffuse reflectance spectra were measured from 300–700 nm with a FLAME-S-UV-VIS spectrometer with an ISP-50-8-R-GT integrating sphere attachment and a UV-VIS-NIR light source (all from Ocean Optics, Duiven, the Netherlands). We used a WS-1-SL Spectralon reflectance standard (Ocean Optics, Duiven, the Netherlands). Multiple trap thicknesses were measured to show the effect of the trap material only and prevent any effect of the background. Translucency was measured as transmittance, which is defined as the proportion of the light energy falling on a body that is transmitted through it. Percentage transmittance spectra were measured with the above spectrometer and light source. The probe and light source were positioned as close as possible to each other on either side of the trap. Absolute irradiance spectra were measured with a FLAME-S-UV-VIS spectrometer calibrated for absolute irradiance (Ocean Optics, Duiven, the Netherlands), with sunlight from a clear sky and the sensor positioned perpendicular to the vertical trap surface at a distance of 50 mm. The side of the trap facing the sensor was defined as the front of the trap and the side away from the sensor was defined as the back. Measurements were taken with the sun shining on the front and with the sun shining on the back.
Flame s uv vis spectrometer
Manufactured by OceanOptics
Sourced in United States
The FLAME-S-UV-VIS spectrometer is a compact and high-performance spectroscopy device designed for a wide range of applications. It offers a wavelength range of 200-850 nm and is capable of performing rapid measurements with high resolution and sensitivity. The spectrometer is built with a robust and reliable design to ensure consistent and accurate results.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using flame s uv vis spectrometer
1
Spectroscopic Characterization of Light Traps
2
Label-free Monitoring of Membrane Formation and Protein Adsorption
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RIfS is a label-free, noninvasive technique determining the optical thickness (n × d) of a thin layer by measuring white light interference. This interference is caused by partial reflection at interfaces whose distance is within the coherence length of white light (43 (link)). RIfS was employed to monitor the formation of SHMs and subsequent protein adsorption to receptor lipid containing membranes in a label-free and time-resolved manner. The experimental setup is described in detail elsewhere (44 (link)). Briefly, a Flame-S-UV/Vis spectrometer (Ocean Optics, Dunedin, FL, USA) was used to record interference spectra at intervals of 2 s. Data were evaluated applying a MATLAB (The MathWorks, Natick, MA, USA) tool following the work of Krick et al. (44 (link)).
3
Spectroscopic Characterization of LEDs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spectroscopic measurements (using FOIS-1 Fiber Optic Integrating Sphere, Ocean Optics; FLAME-S-UV-VIS Spectrometer, Ocean Optics) yielded the emission spectra for the red and NIR LEDs. Exposing the photodetector to light passed through a chopper and recording the photocurrent using a data acquisition system (PXI-1031, National Instruments) provided measurements of the response time.
4
Correlating Optical and Mechanical Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tensile Testing was carried out on a Linkam TST-350 without closing the stress-strain chamber and by using a displacement ramp of 5 mm min−1. The shown values of stress were calculated by considering the constant cross-section area of the specimen’s mid-point at 0% strain. Absorption spectra were measured in transmission with a Flame-S UV-vis spectrometer from Ocean Optics. The spectra were smoothed via Savitzky-Golay filter considering 51 pts (6a ) and 21 pts (6b ). Excitation for photoluminescence (PL) spectra was carried out using a UV LED (Nichia NVSU233A UV SMD-LED, 365 nm, max. 1030 mW, operated with 900 mA) at a distance of 10 cm. The angle between exciting and detected light was 60 degrees. PL spectra were measured on the same spectrometer used for UV-vis spectroscopy and smoothed via Savitzky-Golay filter considering 21 pts. The data of optical properties and mechanical analysis was correlated via measuring time and include a maximum error in the strain of ±2%.
5
Structural and Optical Characterization of CdSe/CdS Quantum Dots
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Structural characterization of the QDs was carried out with a JEOL 2010-F TEM working at 200 kV. UV/Vis absorption spectroscopy of the synthesized CdSe/CdS-QDs was performed employing a Varian Cary 5000 UV-Vis spectrometer. The PL response was recorded using an Ocean Optics Flame-S-UV-Vis spectrometer, using an excitation wavelength of 420 nm. The PL lifetime of the QDs in film was measured using a pulsed laser diode of 405 nm and TCSPC Model in the Fluorolog®−3 system. The J-V curve of the finished solar cell was recorded using an Oriel Sol2A solar simulator under AM1.5 G illumination (100 mW/cm2) at standard testing conditions. EQE characterization was performed with a Newport External Quantum Efficiency Measurement System. Reflectivity measurements were collected on the surface of the solar cells before and after the deposition of the QD film (Varian Cary 5000 UV-Vis spectrometer coupled with an integrating sphere).
6
Ezrin T567D Membrane Binding Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ezrin T567D was recombinantly
expressed in E. coli (BL21(DE3)pLysS,
Novagen, Madison, WI, USA) and purified as described previously.33 (link) RIfS was used to measure the formation of SLBs
on the silicon wafers and binding of the protein onto the membranes.
RIfS is a noninvasive label-free technique to determine optical layer
thicknesses (OT = nd). OT values were monitored using
a flame-S-UV/vis spectrometer (Ocean Optics, Dunedin, FL, USA), recording
a spectrum every 2 s and analyzed utilizing a custom MATLAB script
(R2021a, Mathworks). The experimental setup was described previously.37 (link) After SLB formation, the membrane surface was
rinsed with ezrin buffer and a BSA solution (1 mg/mL in ezrin buffer)
for 5 min. After rinsing again with ezrin buffer for 5 min, ezrin
T567D was added (0.8 μM) for 10 min. Unbound protein was removed
by rinsing with ezrin buffer.
expressed in E. coli (BL21(DE3)pLysS,
Novagen, Madison, WI, USA) and purified as described previously.33 (link) RIfS was used to measure the formation of SLBs
on the silicon wafers and binding of the protein onto the membranes.
RIfS is a noninvasive label-free technique to determine optical layer
thicknesses (OT = nd). OT values were monitored using
a flame-S-UV/vis spectrometer (Ocean Optics, Dunedin, FL, USA), recording
a spectrum every 2 s and analyzed utilizing a custom MATLAB script
(R2021a, Mathworks). The experimental setup was described previously.37 (link) After SLB formation, the membrane surface was
rinsed with ezrin buffer and a BSA solution (1 mg/mL in ezrin buffer)
for 5 min. After rinsing again with ezrin buffer for 5 min, ezrin
T567D was added (0.8 μM) for 10 min. Unbound protein was removed
by rinsing with ezrin buffer.
7
Tensile, Optical, and Photoluminescence Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tensile Testing was carried out on a Linkam TST-350 without closing the stress-strain chamber and by using a displacement ramp of 5 mm min -1 . The shown values of stress were calculated by considering the constant cross-section area of the specimen's mid-point at 0% strain.
Absorption spectra were measured in transmission with a Flame-S UV-vis spectrometer from Ocean Optics. The spectra were smoothed via Savitzky-Golay filter considering 51 pts (4-6a) and 21 pts (4-6b). Excitation for photoluminescence (PL) spectra was carried out using a UV LED (Nichia NVSU233A UV SMD-LED, 365 nm, max. 1030 mW, operated with 900 mA) at a distance of 10 cm. The angle between exciting and detected light was 60 degrees. PL spectra were measured on the same spectrometer used for UV-vis spectroscopy and smoothed via Savitzky-Golay filter considering 21 pts. The data of optical properties and mechanical analysis was correlated via measuring time and include a maximum error in strain of ± 2%.
Absorption spectra were measured in transmission with a Flame-S UV-vis spectrometer from Ocean Optics. The spectra were smoothed via Savitzky-Golay filter considering 51 pts (4-6a) and 21 pts (4-6b). Excitation for photoluminescence (PL) spectra was carried out using a UV LED (Nichia NVSU233A UV SMD-LED, 365 nm, max. 1030 mW, operated with 900 mA) at a distance of 10 cm. The angle between exciting and detected light was 60 degrees. PL spectra were measured on the same spectrometer used for UV-vis spectroscopy and smoothed via Savitzky-Golay filter considering 21 pts. The data of optical properties and mechanical analysis was correlated via measuring time and include a maximum error in strain of ± 2%.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!