Nuclear protein lysate extractions from prostate cancer cells were performed using NE‐PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher Scientific). Total proteins (25 μg) from nuclear or whole‐cell extracts were separated electrophoretically using NuPAGE 4–12% Bis‐Tris gels (Invitrogen), transferred to polyvinylidene difluoride membrane, and hybridized with anti‐OLFM4 (Sino Biological Inc., Wayne, PA), anti‐β‐actin (Santa Cruz Biotechnology, Inc.), anti‐TWIST1 (Novus Biologicals), or anti‐ZEB1, anti‐E‐cadherin, anti‐β‐catenin, antivimentin or antihistone H3 (Cell Signaling Technology, Inc.) antibody overnight at 4°C. Membranes were then incubated with secondary antibody, and signal developed with Amersham ECL Western‐blotting detection reagents (GE Healthcare, Chicago, IL).
Anti olfm4
Manufactured by Sino Biological
Anti‐OLFM4 is a laboratory reagent produced by Sino Biological. It is a specific antibody that can be used to detect and quantify the OLFM4 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Santa Cruz Biotechnology (2 mentions)
Amersham ecl western blotting detection reagent, by GE Healthcare (2 mentions)
Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions)
Anti zeb1, by Cell Signaling Technology (1 mentions)
Anti β catenin, by Cell Signaling Technology (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti histone h3, by Cell Signaling Technology (1 mentions)
Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
Anti vimentin, by Cell Signaling Technology (1 mentions)
Anti shh, by Abcam (1 mentions)
Ptch1, by Abcam (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
2 protocols using anti olfm4
1
Western Blot Analysis of Nuclear Proteins in Prostate Cancer
2
Quantitative Western Blotting of Signaling Proteins
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Samples were separated using 4–12% polyacrylamide gel electrophoresis, transferred to polyvinyldiene fluoride membrane, and hybridized with anti-OLFM4 (Sino Biological Inc.), anti-SHH, PTCH1, GLI1, or GLI2 or anti-SHH, PTCH1, Gli1, or Gli2 (Abcam), anti-β-actin (Santa Cruz Biotechnology, Inc.), or anti-caspase 3 (Cell Signaling Technology, Inc.) antibodies overnight at 4 °C. The membranes were then incubated with secondary antibody and signal developed with Amersham ECL Western-blotting detection reagents (GE Healthcare).
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