Il 10 mm00439616 m1

Manufactured by Thermo Fisher Scientific

The IL-10 Mm00439616_m1 is a laboratory reagent used for gene expression analysis. It is a TaqMan Gene Expression Assay designed to detect and quantify the expression of the mouse interleukin-10 (IL-10) gene.

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Lab products found in correlation

Il 1β mm00434228 m1, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Primer express 2, by Thermo Fisher Scientific (1 mentions) Rnase h, by Promega (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Ifnb1 mm00439552 s1, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

IL-10 (509 citations) Mm00439616_m1 (3 citations)

3 protocols using il 10 mm00439616 m1

Quantitative Analysis of Neuropathic Transcripts

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Total RNA was isolated from sciatic nerves and the lumbar spinal cords using Trizol® reagent (Trifast Eurogold®, Euroclone, Italy) according to manufacturer’s instructions and re-suspended in 10–20 μl of RNases-free water. All procedures were performed as previously described in details [12 (link),22 (link)]. Specific TaqMan probe/primers for mouse Prokineticin 2 (Prok2 Mm 01182450_g1), Prokineticin receptors (Prokr1 Mm00517546_m1; Prokr2 Mm00769571_m1), interleukins (IL-1β Mm00434228_m1; IL-10 Mm00439616_m1) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH Mm99999915_g1) were purchased from Applied Biosystems. Threshold cycle numbers (Ct) of the specific gene of interest and the endogenous control gene GAPDH were determined by ABI PRISM 7000 Sequence Detection System.
The Ct value of the specific gene of interest was normalized to the Ct value of the endogenous control, GAPDH, and the comparative Ct method (2^−ΔΔCt) was then applied using control/ non diabetic group as calibrator.

Castelli M., Amodeo G., Negri L., Lattanzi R., Maftei D., Gotti C., Pistillo F., Onnis V., Congu C., Panerai A.E., Sacerdote P, & Franchi S. (2016). Antagonism of the Prokineticin System Prevents and Reverses Allodynia and Inflammation in a Mouse Model of Diabetes. PLoS ONE, 11(1), e0146259.

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Quantifying Immune Gene Expression in Mice

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RNA was harvested and isolated using RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions. cDNA was synthesised using High Capacity cDNA Reverse Transcription kit (Applied Biosystems), according to the manufacturer’s instructions followed by RNase H (Promega) treatment for 30 min at 37°C. Il10, Il12a, Ifnb1, Oas1g, Stat1, Stat3, Irf7, Irf9 and Tlr4 gene expression were quantified by qRT-PCR (7900HT, Applied Biosystems) using the TaqMan system, and normalised to Hprt1 mRNA. Primer probes used were Il10 (Mm00439616_m1); Il12a (Mm00434165_m1); Ifnb1 (Mm00439552_s1); Oas1g (Mm01730198_m1); Stat1 (Mm_00439518_m1); Stat3 (Mm_01219775_m1); Irf7 (Mm_00516793_g1); Irf9 (Mm_00492679_m1); Tlr4 (Mm0045273_m1); Hprt1 (Mm00446968_m1), all purchased from Applied Biosystems. For the quantification of premature Il10 mRNA, the following primers were designed using Primer Express 2.0 software and custom made by Applied Biosystems – forward (exon 3) 5’AGCATGGCCCAGAAATCAAG-3’; probe (exon 3) 5’CTCAGGATGCGGCTGA-3’; reverse (intron 4) 5’AGAACGCATCTGCTACTCACACA-3’.

Howes A., Taubert C., Blankley S., Spink N., Wu X., Graham C.M., Zhao J., Saraiva M., Ricciardi-Castagnoli P., Bancroft G.J, & O’Garra A. (2016). Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages. The Journal of Immunology Author Choice, 197(7), 2838-2853.

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Quantitative Analysis of Neuroinflammatory Markers

Cited 1 time

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Total RNA was isolated from sciatic nerves, DRG, and the lumbar spinal cords using TRIzol® Reagent (Invitrogen, ThermoFisher Scientific, Italy) according to the manufacturer’s instructions and re-suspended in 10–20 μl of RNase-free water. All procedures were performed as previously described in detail [13 (link), 26 (link)]. Specific TaqMan probes/primers for mouse prokineticin receptors (Prokr1 Mm00517546_m1; Prokr2 Mm00769571_m1), cytokines (IL-1β Mm00434228_m1; IL-6 Mm00446190_m1; TNF-α Mm00443258_m1; IL-10 Mm00439616_m1), CD68 (Mm_03047343), TLR4 (Mm00445274_m1), and glyceraldehydes-3-phosphate dehydrogenase (GAPDH Mm99999915_g1) were purchased from Applied Biosystems. Threshold cycle numbers (Ct) of the specific gene of interest and the endogenous control gene GAPDH were determined by ABI PRISM 7000 Sequence Detection System.
The Ct value of the specific gene of interest was normalized to the Ct value of the endogenous control, GAPDH, and the comparative Ct method (2^−ΔΔCt) was then applied using the control group (vehicle treated mice) as calibrator.

Moschetti G., Amodeo G., Maftei D., Lattanzi R., Procacci P., Sartori P., Balboni G., Onnis V., Conte V., Panerai A., Sacerdote P, & Franchi S. (2019). Targeting prokineticin system counteracts hypersensitivity, neuroinflammation, and tissue damage in a mouse model of bortezomib-induced peripheral neuropathy. Journal of Neuroinflammation, 16, 89.

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