TE cells were washed separately in four drops of 10µl of PBS/PVA solution
and were transferred to a PCR tube with a drop of 0.1µl of PBS solution.
The PCR tubes with the samples were stored at -80°C until they were processed.
The trophectoderm cells were processed for DNA amplification and aCGH analysis
using 24 sure kit and Fluorescent labelling system
(Illumina®), according to the manufacturer's protocols.
Hybridization results were processed and analyzed with the BlueFuse software
(Illumina®). The embryos were further classified according
to their chromosomal constitution. According to aCGH results, the embryos were
classified into diploid (D) or mosaic (M), and further gender identified as f
(female) or m (male). According to this classification, Df and Dm were those
embryos showing diploid female or diploid male homogeneous chromosomal
complements, respectively. Mf and Mm corresponded to mosaic embryos with diploid
female or male cell lines, respectively.
and were transferred to a PCR tube with a drop of 0.1µl of PBS solution.
The PCR tubes with the samples were stored at -80°C until they were processed.
The trophectoderm cells were processed for DNA amplification and aCGH analysis
using 24 sure kit and Fluorescent labelling system
(Illumina®), according to the manufacturer's protocols.
Hybridization results were processed and analyzed with the BlueFuse software
(Illumina®). The embryos were further classified according
to their chromosomal constitution. According to aCGH results, the embryos were
classified into diploid (D) or mosaic (M), and further gender identified as f
(female) or m (male). According to this classification, Df and Dm were those
embryos showing diploid female or diploid male homogeneous chromosomal
complements, respectively. Mf and Mm corresponded to mosaic embryos with diploid
female or male cell lines, respectively.