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Chondrocyte differentiation medium

Manufactured by Merck Group
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Chondrocyte differentiation medium is a cell culture medium designed to promote the differentiation of chondrocytes, which are the cells responsible for the production and maintenance of cartilage. The medium contains a specific combination of nutrients, growth factors, and other components that support the growth and maturation of chondrocytes in vitro.

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Lab products found in correlation

Draq5, by Cell Signaling Technology (1 mentions) 7s nerve growth factor, by Merck Group (1 mentions)

2 protocols using chondrocyte differentiation medium

1

Chondrocyte Differentiation of NRF1 MCF10A

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For differentiation, both an NRF1 MCF10A clone and the vector MCF10A were maintained in a chondrocyte differentiation medium (Sigma, St. Louis, MO, USA) for 28 days. The NRF1 MCF10A clone and vector MCF10A were cultured in either SMGS plus DMEM-F12 or neurobasal media plus nerve growth factor-7S (Sigma), Glutamax, and N2 supplement for a total of 28 days. We determined cell differentiation of NRF1 MCF10A clone at 28 days with staining of Alician blue, α-smooth muscle actin (smooth muscle marker), and β-tubulin III (neuron marker). Nuclei were counterstained with DRAQ5® (Cell Signaling).
Das J.K., Felty Q., Poppiti R., Jackson R.M, & Roy D. (2018). Nuclear Respiratory Factor 1 Acting as an Oncoprotein Drives Estrogen-Induced Breast Carcinogenesis. Cells, 7(12), 234.
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2

Multipotency of Human Mesenchymal Stem Cells

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Multipotency of hMSCs was assessed by inducing the differentiation toward adipogenic, osteogenic, and chondrogenic lineages. We performed differentiation protocols by culturing the cells for 30 days in differentiation mediums: Human Adipocyte Differentiation Medium (Sigma 811D), Human Osteoblast Differentiation Medium (Sigma 417D), and Chondrocyte Differentiation Medium (Sigma 411D).
Hernández-Pérez O.R., Juárez-Navarro K.J., Diaz N.F., Padilla-Camberos E., Beltran-Garcia M.J., Cardenas-Castrejon D., Corona-Perez H., Hernández-Jiménez C, & Díaz-Martínez N.E. (2022). Biomolecules resveratrol + coenzyme Q10 recover the cell state of human mesenchymal stem cells after 1-methyl-4-phenylpyridinium-induced damage and improve proliferation and neural differentiation. Frontiers in Neuroscience, 16, 929590.
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