Tumor tissues were embedded in paraffin and cut into slices prior to IHC staining. The tissues were subjected to antigen retrieval and blocking according the Rabbit SP Detection Kit (Cat#SP9001, ZSGB-BIO). The tissue slices were incubated with 50 µL eEF2, Ki67, TOP1 and TOP2 primary antibodies at 4℃ for 15 h. The slices were then washed and incubated with secondary antibodies at room temperature for 30 min. The slices were then stained with 3,3'-Diaminobenzidine (DAB) working buffer. After re-staining with hematoxylin, the slices were photographed and analyzed using the Aperio ImageScope software.
Rabbit sp detection kit
Manufactured by ZSGB-BIO
Sourced in China
The Rabbit SP Detection Kit is a laboratory product designed for the detection and quantification of Rabbit SP (Substance P) in biological samples. The kit utilizes an enzyme-linked immunosorbent assay (ELISA) method to provide a quantitative measurement of Rabbit SP levels.
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Lab products found in correlation
2 protocols using rabbit sp detection kit
1
IHC Analysis of Tumor Tissue Markers
2
Histological Characterization of Endometrial Tissues
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Eutopic/ectopic endometrial, and UC tissues were fixed in 4% formalin for paraffin section preparation. Each segment was sliced to a thickness of 3 microns. Hematoxylineosin staining was performed to identify the morphological structure of the segments. Additionally, these slides were stained with vimentin or cytokeratin7 (CK7) antibodies using immunohistochemistry to determine the cell types. The rabbit SP detection kit (ZSGB, Beijing, China) was utilized for IHC analysis following the manufacturer's instructions. Antigen retrieval was conducted using sodium citrate buffer (pH 6.0) in a pressure cooker. The slides were incubated overnight at 4°C with primary antibodies against vimentin (1:100 dilution; BS1491; Bio-world Technology) or CK7 (1:100 dilution; 22208-1-AP; Protein tech). PBS was used as a negative control. Chromogenic reaction was achieved using the DAB kit (ZSGB, Beijing, China).
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