AChE (Electron eel type-VI-S) and Aquine BChE along with their substrates acetylthiocholine iodide and butyrylthiocholine iodide, and potassium phosphate buffer (pH 8.0) were purchased from Sigma Aldrich St. Louis, MO, USA. Antioxidant chemicals: DPPH, ABTS, ascorbic acid, malic acid, morin, epigallocatechin gallate, pyrogallol, rutin, quercetin, chlorogenic acid, mandelic acid, hydroxy benzoic acid, and gallic acid along with Folin–Ciocalteu regent were acquired from Sigma Aldrich, Darmstadt, Germany. Donepezil and scopolamine were also purchased from Sigma Aldrich (USA). All these chemicals used were of analytical grade; however, the HPLC solvents were of HPLC grade.
Folin ciocalteu regent
Manufactured by Merck Group
Sourced in United States
The Folin-Ciocalteu reagent is a chemical solution used in colorimetric assays to determine the total phenolic content in various samples, such as plant extracts, food products, and biological samples. It is a complex mixture of phosphomolybdic and phosphotungstic acids. The reagent reacts with phenolic compounds, causing a blue color to develop, which can be measured using a spectrophotometer. The intensity of the color is proportional to the total phenolic content in the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Rutin, by Merck Group (2 mentions)
Epigallocatechin gallate (egcg), by Merck Group (2 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Hydroxybenzoic acid, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Malic acid, by Merck Group (1 mentions)
Pyrogallol, by Merck Group (1 mentions)
Scopolamine, by Merck Group (1 mentions)
Acetylthiocholine iodide, by Merck Group (1 mentions)
Donepezil, by Merck Group (1 mentions)
Potassium phosphate buffer, by Merck Group (1 mentions)
Mandelic acid, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
3 protocols using folin ciocalteu regent
1
Acetylcholinesterase Inhibition Assay
2
Analytical Chemistry Protocol Catalog
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Solvents including hexane, acetone, and acetonitrile were HPLC grade and purchased from Merck (Merck Chemicals, Darmstadt, Germany). Sodium carbonate, Folin-Ciocalteu regent, vanillin regent, sodium nitrite, aluminum chloride, NaOH, glacial acetic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulphonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), gallic acid, ellagic acid, (+)-catechin, (−)-epicatechin, rutin, and EGCG were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Antioxidant Assays and Liver/Kidney Profiles
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Antioxidant chemicals such as DPPH, ABTS, ascorbic acid, antioxidant standards, Folin-Ciocalteu regent, and silymarin, were purchased from Sigma-Aldrich (St. Louis, MO, USA); while normal saline solution (Utsoka Pharma, Las Bela Baluchistan, Pakistan), liver profile test kits (Human, Hamburg, Germany), and kidney profile test kits (Biomed: Germany; diagnostic) of the mentioned firms (in brackets) were used in this study. Methanol was purchased from Merck, Darmstadt, Germany.
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