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Anti ha sg77

Manufactured by Thermo Fisher Scientific
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The Anti-HA SG77 is a laboratory equipment product designed for the detection and analysis of proteins that have been tagged with the hemagglutinin (HA) epitope. It functions as a specific and sensitive tool for the identification and immunoprecipitation of HA-tagged proteins in various experimental applications.

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Lab products found in correlation

Mouse anti flag mab clone m2, by Merck Group (2 mentions) Anti flag 8h8l17, by Thermo Fisher Scientific (2 mentions) Mouse anti exp2 mab clone 7, by Thermo Fisher Scientific (2 mentions) Anti sbp138, by Thermo Fisher Scientific (2 mentions) Anti pfhad139, by Thermo Fisher Scientific (2 mentions) Mouse anti cmyc mab 9e10, by Thermo Fisher Scientific (2 mentions) Anti plasmodium aldolase ab38905, by Abcam (2 mentions) Mouse anti flag monoclonal antibody clone m2, by Merck Group (1 mentions) Ha 11, by Fortrea (1 mentions)

Close spelling variants of this product

Anti-HA

4 protocols using anti ha sg77

1

Antibody Dilutions for IFA and Western Blot

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The following antibodies were used for IFA and western blot at the indicated dilutions: rabbit polyclonal anti-HA SG77 (ThermoFisher) (WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:500, WB 1:500); rabbit mAb anti-FLAG 8H8L17 (ThermoFisher) (WB: 1:500); mouse anti-EXP2 mAb clone 7.736 (WB: 1:1000); mouse anti-HRP2 mAb 2G1237 (IFA 1:500); rabbit polyclonal anti-SBP138 (IFA: 1:500); rabbit polyclonal anti-PfHAD139 (WB: 1:500); mouse anti-cMYC mAb 9E10 (ThermoFisher) (WB: 1:300); rabbit polyclonal anti-Plasmodium Aldolase ab38905 (Abcam) (IFA: 1:1000).
Garten M., Nasamu A.S., Niles J.C., Zimmerberg J., Goldberg D.E, & Beck J.R. (2018). EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX. Nature microbiology, 3(10), 1090-1098.
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2

Antibody Characterization for IFA and WB

Cited 1 time
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The following antibodies were used for IFA and Western blotting at the indicated dilutions: rabbit polyclonal anti-HA SG77 (Thermo Fisher; 1:1,000 [WB]), mouse anti-PbHSP70 monoclonal antibody 4C9 (73 (link)) (1:1,000 [WB]), mouse anti-FLAG monoclonal antibody M2 (Sigma; 1:1,000 [IFA]), goat polyclonal anti-UIS4 (LS Biolabs LS-C204260; 1:1,000 [IFA]), and rabbit polyclonal anti-LISP2 (1:1,000 [IFA]). Anti-LISP2 antiserum was generated by GenScript against the peptide NGQKGNVDEERKSM\, located between the repeat region and 6-Cys domain, a portion of LISP2 previously shown to localize to the PV but not observed to enter the host cell (39 (link)). Purified antiserum labeled the parasite periphery in immunofluorescence assays, and loss of signal following EXP2 knockdown provided validation of specificity.
Hussain T., Linera-Gonzalez J., Beck J.M., Fierro M.A., Mair G.R., Smith R.C, & Beck J.R. (2022). The PTEX Pore Component EXP2 Is Important for Intrahepatic Development during the Plasmodium Liver Stage. mBio, 13(6), e03096-22.
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3

Antibody Dilutions for IFA and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for IFA and western blot at the indicated dilutions: rabbit polyclonal anti-HA SG77 (ThermoFisher) (WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:500, WB 1:500); rabbit mAb anti-FLAG 8H8L17 (ThermoFisher) (WB: 1:500); mouse anti-EXP2 mAb clone 7.736 (WB: 1:1000); mouse anti-HRP2 mAb 2G1237 (IFA 1:500); rabbit polyclonal anti-SBP138 (IFA: 1:500); rabbit polyclonal anti-PfHAD139 (WB: 1:500); mouse anti-cMYC mAb 9E10 (ThermoFisher) (WB: 1:300); rabbit polyclonal anti-Plasmodium Aldolase ab38905 (Abcam) (IFA: 1:1000).
Garten M., Nasamu A.S., Niles J.C., Zimmerberg J., Goldberg D.E, & Beck J.R. (2018). EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX. Nature microbiology, 3(10), 1090-1098.
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4

Antibody Characterization for Immunofluorescence and Western Blot

Cited 1 time
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The following antibodies were used for immunofluorescence assays (IFA) and western blot (WB) analysis at the indicated dilutions: mouse anti-HA monoclonal antibody HA.11 (Covance; 1:500 WB); rabbit polyclonal anti-HA SG77 (ThermoFisher; 1:500 IFA and WB); mouse anti-Flag monoclonal antibody clone M2 (Sigma; 1:300 IFA); mouse anti-EXP1 monoclonal antibody (Lisewski et al., 2014 (link)) (1:500 WB); mouse anti-EXP2 monoclonal antibody clone 7.7 (Hall et al., 1983 (link)) (1:500 IFA and WB); rabbit polyclonal anti-SBP1 (Blisnick et al., 2000 (link)) (1:500 IFA); mouse anti-cMYC monoclonal antibody 9E10 (ThermoFisher; 1:166 IFA and WB); rabbit polyclonal anti-Plasmodium Aldolase ab207494 (Abcam; 1:500 WB); rabbit polyclonal anti-RFP R10367 (ThermoFisher; 1:500 WB).
Nessel T., Beck J.M., Rayatpisheh S., Jami-Alahmadi Y., Wohlschlegel J.A., Goldberg D.E, & Beck J.R. (2020). EXP1 is required for organization of EXP2 in the intraerythrocytic malaria parasite vacuole. Cellular microbiology, 22(5), e13168.
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