Mouse il 1β ready set go kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Mouse IL-1β Ready-Set-Go Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interleukin-1 beta (IL-1β) levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Ldh assay, by Roche (1 mentions)

Close spelling variants of this product

Mouse IL-1β Ready-Set-Go ELISA kit ELISA Ready-Set-Go kit for mouse IL-1β Ready-SET-Go Mouse IL-1β IL-1β kits IL-1β

2 protocols using mouse il 1β ready set go kit

Cytokine and Cell Death Assays

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Supernatants were harvested at various time points for cytokine measurement and in select experiments for the lactate dehydrogenase (LDH) assay (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s protocol. The percentage of LDH release, as a measure of cell death, was calculated as follows: (LDH infected - LDH control)/(LDH total lysis - LDH control) × 100. The remaining supernatants were stored at -20 °C for use in an IL-1β enzyme-linked immunosorbent assay (ELISA) by a mouse IL-1β Ready-Set-Go Kit (eBioscience, San Diego, USA).

Wang X., Gong P., Zhang X., Wang J., Tai L., Wang X., Wei Z., Yang Y., Yang Z., Li J, & Zhang X. (2017). NLRP3 inflammasome activation in murine macrophages caused by Neospora caninum infection. Parasites & Vectors, 10, 266.

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Quantification of IL-1β and IL-18

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Active IL-1β in cell supernatants (collected from 3 × 10⁶ PMs) were measured using the mouse IL-1β Ready-Set-Go Kit, and IL-18 in the mice serum were measured using the mouse IL-18 Ready-Set-Go Kit (eBioscience, San Diego, CA, USA), according to the manufacturer’s instructions.

Li L., Wang X.C., Gong P.T., Zhang N., Zhang X., Li S., Li X., Liu S.X., Zhang X.X., Li W., Li J.H, & Zhang X.C. (2020). ROS-mediated NLRP3 inflammasome activation participates in the response against Neospora caninum infection. Parasites & Vectors, 13, 449.

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