Rat anti e cadherin mab clone decma 1

For immunohistochemistry and immunoblotting, a rat anti-mouse CD9 monoclonal antibody (mAb) (clone KMC8) and mouse anti-human CD9 mAb (clone ALB6) were purchased from BD Biosciences (San Jose, CA), and a mouse anti-mouse vascular endothelial growth factor (VEGF) mAb (clone RM0009-2G02) was purchased from Abcam. For immunohistochemistry, rat anti-integrin α6 and β1, and CD98 mAbs (clones GoH3, KMI6, and H202-141, respectively) were purchased from BD Biosciences, a rat anti-E-cadherin mAb (clone DECMA-1) was purchased from Sigma-Aldrich, and a rabbit anti-HIF-1α polyclonal antibody was purchased from Novus Biologicals. Secondary antibodies for immunohistochemistry were Alexa Fluor 488- and 546-conjugated IgGs purchased from Molecular Probes. Horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) were used for immunoblotting. Nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI) (WAKO Pure Chemical Industries).