Morphine, hydromorphone, oxymorphone, butorphanol, levorphanol, nalbuphine, nalorphine, naltrexone, 6β-naltrexol, buprenorphine, pentazocine, and naloxone, were purchased from Cerilliant. Adenosine 5’-triphosphate (ATP), sodium dodecyl sulfate (SDS), dithiothreitol (DTT), dimethyl sulfoxide (DMSO), 3-(N-morpholino)propanesulfonic acid (Mops), Trizma base (Tris), isopropyl-β-D-thiogalactopyranoside (IPTG), and silica gel thin-layer chromatography (TLC) plates were from Sigma Chemical Company. Polyethyleneimine (PEI) cellulose TLC plates were from Brinkmann. Ecolume scintillation cocktail was purchased from MP Biomedical. Carrier-free sodium [35S]sulfate was from PerkinElmer. HepG2 human hepatoma cell line (ATCC HB-8065) was obtained from American Type Culture Collection. Pooled human lung S9 fraction, liver cytosol, small intestine (duodenum and jejunum) S9 fraction, and kidney S9 fraction were purchased from XenoTech, LLC. All other chemicals were of the highest grade commercially available.
Butorphanol
Manufactured by Cerilliant
Sourced in United States
Butorphanol is a synthetic opioid analgesic compound used in research and laboratory settings. It acts as a partial agonist at the mu-opioid receptor and an antagonist at the kappa-opioid receptor. Butorphanol is commonly used in studies involving pain management and analgesia.
Automatically generated - may contain errors
Lab products found in correlation
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
Carrier free sodium 35s sulfate, by PerkinElmer (1 mentions)
Adenosine 5 triphosphate, by Merck Group (1 mentions)
Silica gel thin layer chromatography, by Merck Group (1 mentions)
3 n morpholino propanesulfonic acid, by Merck Group (1 mentions)
Morphine, by Cerilliant (1 mentions)
Isopropyl β d thiogalactopyranoside, by Merck Group (1 mentions)
Nalorphine, by Cerilliant (1 mentions)
Naltrexone, by Cerilliant (1 mentions)
Naloxone, by Cerilliant (1 mentions)
Oxymorphone, by Cerilliant (1 mentions)
Hydromorphone, by Cerilliant (1 mentions)
Buprenorphine, by Cerilliant (1 mentions)
Ecolume scintillation cocktail, by MP Biomedicals (1 mentions)
Pooled human lung s9 fraction, by Xenotech (1 mentions)
Liver cytosol, by Xenotech (1 mentions)
Small intestine duodenum and jejunum s9 fraction, by Xenotech (1 mentions)
2 protocols using butorphanol
1
Opioid Receptor Binding Assay
2
Chemical Sources for Pharmacological Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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(-)-Mitragynine (7) was obtained from Chiron (Trondheim, Norway). 2-methyl-AP-237 HCl (1), AP-237 HCl (2), trans-bromadol (3), brorphine HCl (4), isotonitazene (6), 7-OH-mitragynine (8) and piperidylthiambutene HCl (10) were purchased from Cayman Chemical Company (Ann Arbor, Michigan, US). Butorphanol (5), MT-45 (9) (as racemic mixture) and tianeptine (11) were kind gifts from Prof. V. Auwärter (University of Freiburg) and were originally obtained from Cerilliant (Round Rock, Texas, US), Lipomed AG (Arlesheim, Switzerland) and Cayman Chemical Company (Ann Arbor, Michigan, US), respectively. Hydromorphone (A) was purchased as hydrochloride salt from Fagron (Nazareth, Belgium). Fentanyl (B) was obtained from LGC Chemicals (Wesel, Germany). Dulbecco's modified Eagle's medium (DMEM; GlutaMAX™), Opti-MEM® I reduced serum medium, penicillinstreptomycin (5 000 U/mL) and amphotericin B (250 µg/mL) were obtained from Thermo Fisher Scientific (Pittsburg, PA, USA). Fetal bovine serum (FBS) and poly-D-lysine were supplied by Sigma Aldrich (Overijse, Belgium). The Nano-Glo® Live Cell Assay system (containing the Nano-Glo® Live Cell Substrate and Nano-Glo® LCS Dilution Buffer) was procured from Promega (Madison, WI, USA).
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