Log In Sign up
The largest database of trusted experimental protocols

Dubeccos modified eagle medium

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in Germany

Dulbecco's Modified Eagle Medium (DMEM) is a commonly used basal medium for the in vitro cultivation of various cell types, including mammalian cells. DMEM provides a balanced salt solution and a variety of nutrients necessary for cell growth and maintenance.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (2 mentions) Svec4 10, by American Type Culture Collection (2 mentions) Renca, by American Type Culture Collection (2 mentions) Hepg2, by American Type Culture Collection (2 mentions) Live dead assay kit, by Thermo Fisher Scientific (2 mentions) Gold 3 chloride trihydrate, by Merck Group (2 mentions) L glutathione reduced, by Merck Group (2 mentions) Dulbecco s modified eagle medium ham s f12, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Adrenaline, by AstraZeneca (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Amaxa nucleofector 2b, by Lonza (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Selenocystamine dihydrochloride, by Merck Group (1 mentions)

6 protocols using dubeccos modified eagle medium

1

Culturing Breast Cancer and Normal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human breast adenocarcinoma cells (MCF-7 ATCC # HTB-22) were cultured in Dubeccos Modified Eagle Medium (Gibco) with 10% fetal bovine serum and 1% penicillin-streptomycin. Human breast cells (MCF-10A ATCC # CRL-10317) were cultured in Dulbecco’s Modified Eagle Medium/Ham’s F-12 (Gibco) with 5% horse serum, 20 ng/mL EGF, 0.5 mg/mL hydrocortisone, 100 ng/mL cholera toxin, 10 μg/mL insulin, and 1% penicillin streptomycin. Cells were treated with 0.05% trypsinEDTA (Gibco) for seeding on the sensor through a 100 μL well at a density of 9,000 cells per 100 μL.
Corbin E.A., Dorvel B.R., Millet L.J., King W.P, & Bashir R. (2014). Micro-patterning of Mammalian Cells on Suspended MEMS Resonant Sensors for Long-Term Growth Measurements. Lab on a chip, 14(8), 1401-1404.
+ Open protocol
+ Expand
2

Generation of Human Induced Pluripotent Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Skin biopsies were performed under local anaesthetic (Xylocaine 2% with Adrenaline 100 micrograms/20 ml (1:200,000), AstraZeneca) using a sterile technique following ethical approval (REC Ref No 10/S1103/10, Lothian R&D Project No 2012/R/DER/01) and informed consent. Both patients and controls were aware of their use of their samples in research. Fibroblast cell lines developed in Dubecco’s Modified Eagle Medium (Gibco) with 10% fetal calf serum (Gibco) and 1:500 Penicillin/Streptomycin (Gibco).
Three micrograms of expression plasmid mixtures (generous donation from Yamanaka lab)9 (link) were electroporated into 500,000 fibroblasts in Amaxa Nucleofector 2b (Lonza AAB1001) according to the manufacturer’s instructions and transferred to a 6-well Gelatin-coated plate (1% Sigma) in DMEM/10% FCS. The cells were trypsinised upon reaching confluence and replated on Geltrek in Essential8 media43 (E8; Gibco) in a 100 ml dish. Colonies were counted after 25 days and hESC-looking colonies picked and expanded. Cells were maintained on Geltrek in E8 and passaged with EDTA (0.5 M, Invitrogen).
Megaw R., Abu-Arafeh H., Jungnickel M., Mellough C., Gurniak C., Witke W., Zhang W., Khanna H., Mill P., Dhillon B., Wright A.F., Lako M, & ffrench-Constant C. (2017). Gelsolin dysfunction causes photoreceptor loss in induced pluripotent cell and animal retinitis pigmentosa models. Nature Communications, 8, 271.
+ Open protocol
+ Expand
3

Cell Culture Protocols for Diverse Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vero cells (African green monkey kidney) were maintained in OPTI-MEM (Gibco) cell culture medium, containing 3% fetal bovine serum (FBS, Bovogen) at 37 °C with 5% CO2. C6/36 (Aedes albopictus) cells were grown in glutamate-free Roswell Memorial Park Institute-1640 (RPMI, Gibco) medium, supplemented with 10% FBS, 15 mM HEPES (Gibco), 1% GlutaMAXTM (Gibco), 100 U/mL penicillin (Gibco) and 100 μg/mL streptomycin (Gibco) at 28 °C. K-562 cells (human bone marrow) were maintained in RPMI-1640, supplemented with 10% FBS, 100 U/mL penicillin (Gibco) and 100 μg/mL streptomycin (Gibco) at 37 °C with 5% CO2. A549 cells (human lung epithelial) were maintained in Dubecco’s modified Eagle medium (Gibco), supplemented with 10% FBS, 100 U/mL penicillin (Gibco) and 100 μg/mL streptomycin (Gibco) at 37 °C with 5% CO2.
Choo J.J., Vet L.J., McMillan C.L., Harrison J.J., Scott C.A., Depelsenaire A.C., Fernando G.J., Watterson D., Hall R.A., Young P.R., Hobson-Peters J, & Muller D.A. (2021). A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice. NPJ Vaccines, 6, 66.
+ Open protocol
+ Expand
4

Nanoparticle-based Doxorubicin Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gold(III) chloride trihydrate, sodium borohydride, poly(bis(4-carboxyphenoxy)phosphazene) disodium salt (PCPP, 1 MDa), L-glutathione reduced (GSH), selenocystamine dihydrochloride, hexamethylenediamine, doxorubicin and albumin-fluorescein conjugate were purchased from Sigma-Aldrich (St. Louis, MO). Quisinostat dihydrochloride was purchased from Selleckchem (Houston, TX). HepG2, U251, Renca, and SVEC4-10 cell lines were purchased from ATCC (Manassas, VA). LIVE/DEAD assay kits were purchased from Life Technologies Invitrogen (Grand Island, NY). Cells were cultured in Dubecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum and 1% penicillin (10000 units/mL) from Life Technologies Invitrogen (Grand Island, NY). Athymic nude mice were obtained from Charles River Laboratories (stock no. 490).
Bouché M., Cormode D.P., Dong Y.C., Sheikh S., Taing K., Saxena D., Hsu J.C., Chen M.H., Salinas R.D., Song H., Burdick J.A, & Dorsey J. (2021). Novel Treatment for Glioblastoma Delivered by a Radiation Responsive and Radiopaque Hydrogel. ACS biomaterials science & engineering, 7(7), 3209-3220.
+ Open protocol
+ Expand
5

Establishing Neural Stem Cell and Medulloblastoma Cell Lines

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
DKFZ-EP1NS [25 (link)], SJ-BT57 [52 (link)], and human fetal neural stem cells obtained from C. Watts, University of Cambridge were cultivated as neural stem cells using neurosphere medium (Invitrogen, Karlsruhe, Germany) with growth factor supplements as described [25 (link)] Medulloblastoma cells DAOY (obtained from American Type Culture Collection) and UW228-3 cells (obtained from Steven Clifford, Newcastle, United Kingdom), were cultivated adherently using normal DMEM (Dubecco's modified Eagle medium, Invitrogen, Karlsruhe, Germany). Based on DNA methylation profiling using Illumina 450k methylation array [11 (link)], cell lines EP1NS and SJ-BT57 were classified as preclinical RELA-positive (EP1NS) and posterior fossa Group A (SJ-BT57) models. Additionally, according to the study of Parker and colleagues [15 (link)], the RELA-C11orf95 fusions was detected in EP1NS by reverse-transcription PCR, detailed description can be found elsewhere [11 (link), 15 (link)].
Tzaridis T., Milde T., Pajtler K.W., Bender S., Jones D.T., Müller S., Wittmann A., Schlotter M., Kulozik A.E., Lichter P., Collins V.P., Witt O., Kool M., Korshunov A., Pfister S.M, & Witt H. (2016). Low-dose Actinomycin-D treatment re-establishes the tumoursuppressive function of P53 in RELA-positive ependymoma. Oncotarget, 7(38), 61860-61873.
+ Open protocol
+ Expand
6

Synthesis and Characterization of Arylboronate Polyphosphazene

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gold(III) chloride trihydrate, sodium borohydride,
poly-di-(carboxylatophenoxy)phosphazene disodium salt (PCPP, 1 MDa), spermine,
reduced l-glutathione, hydrogen peroxide (H2O2),
ferrous perchlorate hydrate
(Fe(ClO4)2·xH2O),
potassium dioxide (KO2), and lipopolysaccharides (LPS) were purchased
from Sigma-Aldrich (St. Louis, MO). PEG-PLL (PEG MW 5000/PLL
MW 4900) was purchased from Alamanda Polymers. Recombinant human
interferon-γ protein (IFN-γ) was
purchased from Abcam. For the microfluidic setup, the herringbone mixer microfluidic
chip (channel diameter of 600 μm) and male luers were
purchased from Microfluidic ChipShop (Jena, Germany), the polyethylene tubing from
VWR (Philadelphia, PA), and the syringe pumps from Braintree Scientific (Braintree,
Ma). HepG2, RAW264.7, Renca, and SVEC4–10 cell lines were purchased from ATCC
(Manassas, VA). LIVE/DEAD assay kits were purchased from Invitrogen (Grand Island,
NY). Cells were cultured in Dubecco’s Modified Eagle Medium from Invitrogen
(Grand Island, NY) supplemented with 1% penicillin and 10% fetal bovine serum unless
specified otherwise. TNF-α ELISA kit was purchased from
Invitrogen (Grand Island, NY).
The experimental details for the synthesis of the arylboronate
polyphosphazene derivative (PPB) are detailed in the Supporting Information.
Bouché M., Pühringer M., Iturmendi A., Amirshaghaghi A., Tsourkas A., Teasdale I, & Cormode D.P. (2019). Activatable Hybrid Polyphosphazene-AuNP Nanoprobe for ROS Detection by Bimodal PA/CT Imaging. ACS applied materials & interfaces, 11(32), 28648-28656.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!