Mccoy s media

HEK293, HeLa and MDCK cells were cultured in Dulbecco's modified Eagle medium (DMEM; Wisent) media supplemented with 10% Cosmic calf serum (CCS; Cytoviva). HCT116 cells were cultured in McCoy's media (Wisent) supplemented with 10% CCS. HepG2 cells were cultured in Eagle's minimum essential medium (EMEM; Wisent) supplemented with 10% fetal bovine serum (FBS; Cytoviva). The cells were maintained in 10 cm dishes in incubators at 37°C with 5% CO₂ as per standard protocols [38 (link),39 (link)]. For long-term storage, cells were washed and resuspended in freezing media (50% FBS, 40% DMEM or EMEM media and 10% DMSO) and stored in liquid nitrogen.

HeLa and HFF-1 cells obtained from the American Type Culture Collection (ATCC) were grown in DMEM (Wisent), while A549 cells were grown in F12K media (Wisent) and HCT 116 (p53-/-) cells were grown in McCoy’s media (Wisent) in humidified incubators at 37 °C with 5% CO₂. All media were supplemented with 10% fetal bovine serum (FBS; Thermo Scientific), 2 mM l-glutamine (Wisent), 100 U penicillin (Wisent), and 0.1 mg/mL streptomycin (Wisent).
C87 and C75 were stored at − 20 °C as 1 mM stocks in dimethyl sulfoxide (DMSO). Working stocks of 100 μM C75 or C87 were made in DMSO:H₂O (9:1). Colchicine (Sigma) was dissolved in ethanol as a 1 M stock and diluted to 10 μM before use. Nocodazole was dissolved in DMSO as a 1 mg/mL stock (Sigma). Paclitaxel (Bioshop) was dissolved in DMSO as a 10 mM stock and diluted to a range of smaller concentrations before use. Cells grown as monolayer cultures or as spheroids were treated with C75, C87, Colchicine, or paclitaxel as indicated, and the final concentrations of DMSO or ethanol were kept below 0.5%.