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15n4 arg

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15N4-ARG is a stable isotope-labeled arginine product offered by Cambridge Isotopes. It contains four nitrogen-15 atoms, making it a useful tool for research and analytical applications that require nitrogen-15 labeling.

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Lab products found in correlation

Cell culture reagents, by Thermo Fisher Scientific (2 mentions) Bovine brain calmodulin, by Merck Group (1 mentions) L ng nitroarginine methyl ester l name, by Merck Group (1 mentions) Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) L citrulline, by Cambridge Isotopes (1 mentions) 15n nitrite, by Cambridge Isotopes (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (1 mentions) 13c6 arg, by Cambridge Isotopes (1 mentions) D7 adma, by Cambridge Isotopes (1 mentions)

2 protocols using 15n4 arg

1

Nitric Oxide Synthesis Assay Protocol

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ARG [as L-arginine HCl], CIT [as L-citrulline], 4-(2-Hydroxyethyl)-1 piperazineethanesulfonic acid (HEPES), CaCl2·4H2O, bovine brain calmodulin, (6R)-5,6,7,8-tetrahydro-l-biopterin (BH4), NADPH, ethylene diamine tetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), and L-NG-Nitroarginine methyl ester (L-NAME) were purchased from Sigma (St. Louis, MO). 15N4-ARG [as its HCl, (U-15N4, 98%)], the internal standard for ARG, 13C6-ARG [as ARG:HCl (U-13C6, 98%)], the internal standard for CIT, D4-CIT [as L-citrulline (4,4,5,5-D4, 96.5 %)], and 15N-nitrite [as NaNO2 (15N, 98%+)] were obtained from Cambridge Isotope Laboratories, Inc. These compounds were used without further purification. Cell culture reagents were purchased from Invitrogen.
Shin B.S., Fung H.L., Upadhyay M, & Shin S. (2015). Estimation of nitric oxide synthase activity via LC-MS/MS determination of 15N3-citrulline in biological samples. Rapid communications in mass spectrometry : RCM, 29(5), 447-455.
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2

Arginine and Dimethylarginine Quantification

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ARG [as L-arginine HCl], ADMA [as NG,NG-dimethylarginine dihydrochloride], and SDMA [as NG,NG′-dimethyl-L-arginine di(p-hydroxyazobenzene-p′-sulfonate) salt] were purchased from Sigma. Stable isotope-labeled compounds, 15N4-ARG [as its HCl, (U-15N4, 98%)], 13C6-ARG [as ARG:HCl (U-13C6, 98%)], and D7-ADMA [as ADMA:HCl:H2O (2,3,3,4,4,5,5-D7, 98%)] were obtained from Cambridge Isotope Laboratories, Inc. These compounds were used without further purification. Cell culture reagents were purchased from Invitrogen.
Shin S., Thapa S.K, & Fung H.L. (2017). Cellular interactions between L-arginine and asymmetric dimethylarginine: Transport and metabolism. PLoS ONE, 12(5), e0178710.
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