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Plenti ddk puro egfp vector

Manufactured by Addgene
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PLENTI-DDK-puro-eGFP vector is a plasmid designed for the expression of proteins tagged with a DDK (Dual-Strep-FLAG) tag and eGFP (enhanced green fluorescent protein) in mammalian cells. The vector contains a puromycin resistance gene for selection of transfected cells.

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Lab products found in correlation

Pmirglo luciferase expression vector, by Promega (2 mentions) Lenticrispr v2 blast vector, by Addgene (2 mentions) Lenticrispr v2 vector, by Addgene (2 mentions)

Close spelling variants of this product

PLenti-puro vector PLenti-puro

2 protocols using plenti ddk puro egfp vector

1

Molecular Cloning for Genetic Manipulation

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shRNAs were cloned into the pHIV7-GFP lentiviral vector. sgRNAs were cloned into lentiCRISPR v2 vector (Addgene plasmid # 52961) or lentiCRISPR v2-Blast vector (Addgene plasmid # 83480). The sequences for shRNAs and sgRNAs were listed in Supplemental table 11. The WT or mutant PUS7 (D256A)47 was cloned into the CSC lentiviral vector. The reporter plasmids were prepared by inserting 6 x CGG or 6 x CGA sequences before the firefly luciferase coding region in the pmirGlo luciferase expression vector (Promega) to obtain the 6 x Arg (CGG) or the 6 x Arg (CGA) reporter plasmid. The TYK2 fragment plasmids were cloned by replacing the eGFP sequences in the pLENTI-DDK-puro-eGFP vector (Addgene plasmid #123299) with the WT or mutant TYK2 fragment (aa 100- aa 264).
Cui Q., Yin K., Zhang X., Ye P., Chen X., Chao J., Meng H., Wei J., Daniel R., Li L., Qin Y., Sun G., Zhang M., Klein J., Huynhle M., Wang C., Zhang L., Badie B., Kalkum M., He C., Yi C, & Shi Y. (2021). Targeting PUS7 suppresses tRNA pseudouridylation and glioblastoma tumorigenesis. Nature cancer, 2(9), 932-949.
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2

Molecular Cloning for Genetic Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
shRNAs were cloned into the pHIV7-GFP lentiviral vector. sgRNAs were cloned into lentiCRISPR v2 vector (Addgene plasmid # 52961) or lentiCRISPR v2-Blast vector (Addgene plasmid # 83480). The sequences for shRNAs and sgRNAs were listed in Supplemental table 11. The WT or mutant PUS7 (D256A)47 was cloned into the CSC lentiviral vector. The reporter plasmids were prepared by inserting 6 x CGG or 6 x CGA sequences before the firefly luciferase coding region in the pmirGlo luciferase expression vector (Promega) to obtain the 6 x Arg (CGG) or the 6 x Arg (CGA) reporter plasmid. The TYK2 fragment plasmids were cloned by replacing the eGFP sequences in the pLENTI-DDK-puro-eGFP vector (Addgene plasmid #123299) with the WT or mutant TYK2 fragment (aa 100- aa 264).
Cui Q., Yin K., Zhang X., Ye P., Chen X., Chao J., Meng H., Wei J., Daniel R., Li L., Qin Y., Sun G., Zhang M., Klein J., Huynhle M., Wang C., Zhang L., Badie B., Kalkum M., He C., Yi C, & Shi Y. (2021). Targeting PUS7 suppresses tRNA pseudouridylation and glioblastoma tumorigenesis. Nature cancer, 2(9), 932-949.
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