Elecsys nse cobas e801

The data on brain-specific biomarkers, NSE level, at 24 h after the ROSC were used for this analysis. All serum samples were obtained from an arterial catheter and analyzed in one laboratory, namely the Green Cross Laboratory (GC Labs; Yongin, Gyeonggi-do, Korea). The NSE concentration was determined using an electrochemiluminescence immunoassay with Elecsys NSE^® (COBAS e801; Roche Diagnostics, Basel, Switzerland). The measurement range of the NSE level was 0.1–300 ng/mL. Finally, GC Labs was compliant with the relevant national and international guidelines.

Serum NGAL and NSE levels were obtained from blood samples drawn by an arterial line at 24-hour intervals until 72 hours after ROSC (ie, NGAL_{initial, 24, 48, and 72} and NSE_{initial, 24, 48, and 72}). The first NGAL and NSE measurements were obtained at the time of applying an external cooling device after several procedures for TTM, including electrocardiography, echocardiography, brain imaging, or resuscitation for sustained ROSC. NGAL and NSE values were measured using a UniCel DxC 880i chemical analyzer (Beckman Coulter Inc., Brea, CA) with the NGAL Test kit (BioPorto Diagnostics, Gentofte, Denmark) and an electrochemiluminescence immunoassay with Elecsys NSE (COBAS e801; Roche Diagnostics, Rotkreuz, Switzerland). The NGAL and NSE measurements were in the ranges of 25 to 3000 ng/mL and 0.1 to 300 ng/mL, respectively.