Cells were washed once with PBS 1X and then fixed with paraformaldehyde (PFA) 4% pH 7.4 15 min at RT. After three washes with PBS 1X, permeabilization with PBS/Triton-X-100 0.3% was performed for 5 min at RT. Next, cells were saturated with blocking solution (FBS 5% in PBS/Triton-X-100 0.3%) for 1 h at RT and incubated with primary antibody anti-β amyloid clone 6E10 (Biolegend 803004, 1:50), anti-GFAP (Invitrogen 13-0300, 1:500) and anti-CD11b (BD Biosciences 550282, 1:100) diluted in blocking solution. After three washes in PBS 1X, cells were incubated with secondary antibody AlexaFluor 488 or 594 (ThermoFisher Scientific, 1:500) for 1 h at RT. After several washes in PBS 1X, cells were mounted using Prolong Gold Antifade reagent containing DAPI (ThermoFisher Scientific). Images were acquired with a Zeiss Axioplan2 fluorescence microscope with a 63× oil immersion objective (Carl Zeiss AG, Oberkocken, Germany).
Anti β amyloid clone 6e10
Manufactured by BioLegend
Anti-β amyloid clone 6E10 is a mouse monoclonal antibody that recognizes the N-terminus of amyloid-beta (Aβ) peptide. It can be used to detect and quantify Aβ in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Axioplan 2 fluorescence microscope, by Zeiss (1 mentions)
Alexa fluor 488 or 594, by Thermo Fisher Scientific (1 mentions)
Anti gfap, by Thermo Fisher Scientific (1 mentions)
Prolong gold anti fade reagent containing dapi, by Thermo Fisher Scientific (1 mentions)
Anti cd11b, by BD (1 mentions)
Anti pkcα, by Abcam (1 mentions)
Anti rpe65, by Novus Biologicals (1 mentions)
Anti ripk3, by Santa Cruz Biotechnology (1 mentions)
Anti p mlkl, by Abcam (1 mentions)
Anti s opsin, by Merck Group (1 mentions)
Anti m opsin, by Merck Group (1 mentions)
Anti p21, by Santa Cruz Biotechnology (1 mentions)
Anti zo 1, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Anti ctbp2, by BD (1 mentions)
2 protocols using anti β amyloid clone 6e10
1
Immunofluorescence Analysis of Alzheimer's Markers
2
Comprehensive Immunohistochemical Analysis of Retina and Brain
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cross-sections of the retina or brain and whole-mount retinas for immunohistochemical analysis were firstly washed several times with PBS, and then blocked in PBS containing 5% goat serum and 1% Triton x-100 for 1h at room temperature. The blocked tissues were incubated with the following primary antibodies overnight at 4°C: anti-β-amyloid, clone 6E10 (1:500; BioLegend), anti-β-amyloid, clone 4G8 (1:500; BioLegend), anti-rhodopsin, clone 1D4 (1:1000; Santa Cruz), anti-M-opsin (1:500; Millipore), anti-S-opsin (1:500; Millipore), anti-PKCα (1:200; Abcam), anti-PKCα (1:100; Invitrogen), anti-CtBP2 (1:200; BD), anti-PSD95 (1:100; CST), anti-ZO-1 (1:100; Invitrogen), anti-RPE65 (1:200; Novus), anti-p16ink4α (1:100; Proteintech), anti-p21 (1:100; Santa Cruz), anti-cleaved caspase3 (1:100; CST), anti-RIPK3 (1:100; Santa Cruz), anti-pRIPK3 (1:400; CST) and anti-pMLKL (1:100; Abcam). AlexFluro-488 or -555 conjugated secondary antibody (1:500; CST) incubation were performed for 2.5 h at room temperature. Thereafter, DAPI (1:1000, Invitrogen) were used to label the nuclear for 10 min at room temperature.
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