Normal human dermal fibroblasts (HFIB) were acquired from PromoCell (Labclinics, S.A., Barcelona, Spain). T-flasks, cell culture plates and other cell culture consumables were obtained from ThermoFisher Scientific (Porto, Portugal). Cell imaging plates were bought from Ibidi GmbH (Ibidi, Munich, Germany). Fetal bovine serum (FBS) was purchased from Biochrom AG (Berlin, Germany). PI was procured from Invitrogen (Carlsbad, CA, USA). Agarose was obtained from Grisp (Porto, Portugal). Dulbecco’s modified Eagle’s medium F12 (DMEM-F12), paraformaldehyde (PFA), phosphate-buffered saline (PBS), trypsin, formamide (≥99.5%), ethylenediaminetetraacetate (EDTA), and PEG 4000 Da were purchased from Sigma-Aldrich (Sintra, Portugal).
Ethylenediaminetetraacetate edta
Manufactured by Merck Group
Sourced in Portugal, Macao, United States
Ethylenediaminetetraacetate (EDTA) is a chemical compound commonly used in laboratory settings. It functions as a chelating agent, capable of binding to metal ions and forming stable complexes. EDTA is widely utilized in various analytical and purification techniques to control the presence and behavior of metal ions in the test environment.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture plates, by Thermo Fisher Scientific (1 mentions)
T flasks, by Thermo Fisher Scientific (1 mentions)
Normal human dermal fibroblasts hfib, by PromoCell (1 mentions)
Cell imaging plates, by Ibidi (1 mentions)
Formamide 99.5, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions)
Anti cd14 magnetic bead, by Miltenyi Biotec (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
Gm csf, by Thermo Fisher Scientific (1 mentions)
Human serum, by Merck Group (1 mentions)
Hek293t cells, by Thermo Fisher Scientific (1 mentions)
Human ab serum, by Merck Group (1 mentions)
Ferrous chloride, by Merck Group (1 mentions)
Vanillin, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Fujifilm (1 mentions)
Ferrozine, by Merck Group (1 mentions)
Glucose c6h12o6, by Fujifilm (1 mentions)
Butylated hydroxyanisole, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
4 protocols using ethylenediaminetetraacetate edta
1
Culturing Normal Human Dermal Fibroblasts
2
Culturing and Differentiating Human Cell Lines
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HEK293T cells (Invitrogen, Carlsbad, CA) and the HeLa cell line TZM-bl (Obtained through NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH, from John C Kappes, Xiaoyun Wu and Tranzyme (Catalogue No. 8129)) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Waltham, MA) supplemented with 10% fetal calf serum (FCS) (Gibco).
Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C. MDDCs were generated by culturing purified monocytes in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% fetal calf serum (FCS), IL-4 (1,000 U/ml) and GM-CSF (1,000 U/ml) (Biosource, Carlsbad, CA) for 24 hours. MDMs were made by plastic adherence of monocytes in serum free media for 1 hour before supplemented with RPMI 1640 media containing human AB serum (Sigma Aldrich), which brought the final concentration of human serum to 10%. MDMs were used after 7 days of differentiation for experiments.
Monocytes were purified from peripheral blood mononuclear cells by positive selection using anti-CD14 magnetic beads as outlined by the manufacturer (Miltenyi Biotech, Gladbach, Germany) with the exception of using phosphate-buffered saline (PBS) supplemented with 1% (v/v) human AB serum (Sigma Aldrich, St Louis, MO) and 1 mmol/l ethylenediaminetetraacetate (EDTA) (Sigma Aldrich) at 4°C. MDDCs were generated by culturing purified monocytes in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% fetal calf serum (FCS), IL-4 (1,000 U/ml) and GM-CSF (1,000 U/ml) (Biosource, Carlsbad, CA) for 24 hours. MDMs were made by plastic adherence of monocytes in serum free media for 1 hour before supplemented with RPMI 1640 media containing human AB serum (Sigma Aldrich), which brought the final concentration of human serum to 10%. MDMs were used after 7 days of differentiation for experiments.
3
Quantification of Antioxidants in Soybean and Adlay
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All of the solvents and chemicals used were of analytical grade. Sodium hydroxide (NaOH), 95% ethanol, 99% methanol, phenol, hydrochloric acid (HCl), magnesium sulfate (MgSO4·7H2O), dipotassium hydrogen phosphate (K2HPO4), and glucose (C6H12O6) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Potato dextrose agar (PDA) and potato liquid broth (PDB) were purchased from Difco Co., Ltd. (Sparks, MD, USA). Concentrated sulfuric acid (H2SO4) and acetic acid (CH3COOH) were purchased from Union Chemical Works, Ltd. (Taipei, Taiwan). Ergosterol standards, Folin–Ciocalteu reagent, quercetin, gallic acid, oleanolic acid, vanillin, perchloric acid, 1,1-diphenyl-2-picryl hydrazyl (DPPH), ascorbic acid, butylated hydroxyanisole (BHA), ferrozine, ferrous chloride (FeCl2‧4H2O), ethylene diamine tetra acetate (EDTA), potassium ferricyanide, trichloroacetic (TCA), ferric chloride, diclofenac yellow acetate (2′, 7′-dichlorofluorescin, DCFDA-DA), and acridine orange dye were purchased from Sigma Chemical Co., Ltd. (St. Louis, MO, USA). HPLC-grade 100% methanol was purchased from Labscan Asia Co., Ltd. (Thailand). Soybean and red adlay were purchased from a local market in Yilan City, Taiwan.
4
Synthesis of Organometallic Compounds
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Cupric chloride (CuCl2), (Merck, India), ferric chloride (FeCl3) (Sigma Aldrich, USA), Ammonia (Sigma Aldrich, USA), thiophene (Sigma Aldrich, USA), tert-butanol (TBA) (Merck, India), disodium sulfate (Na2SO4) (Sigma Aldrich, USA), ethylene diamine tetra acetate (EDTA) (Sigma Aldrich, USA), sodium nitrate (Sigma Aldrich, USA), ethanol (Merck, India), chloroform (Merck, India), were used without further purification. Urea (CH4N2O) (molar mass 60.06 g/mol) (Merck, India) was used as received.
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